Difference between revisions of "YxjG"
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| − | * '''Description:''' putative methionine synthase <br/><br/> | + | * '''Description:''' putative B12-independent methionine synthase <br/><br/> |
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
** S-bacillithiolation upon hypochlorite stress on Cys-346 {{PubMed|21749987}} | ** S-bacillithiolation upon hypochlorite stress on Cys-346 {{PubMed|21749987}} | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' | + | * '''Structure:''' [http://pdb.org/pdb/explore/explore.do?structureId=1ypx 1YPX] (the enzyme from ''Listeria monocytogenes'', 49% identity) |
* '''UniProt:''' [http://www.uniprot.org/uniprot/P42318 P42318] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P42318 P42318] | ||
Revision as of 15:37, 16 March 2014
- Description: putative B12-independent methionine synthase
| Gene name | yxjG |
| Synonyms | |
| Essential | no |
| Product | putative methionine synthase |
| Function | unknown |
| Gene expression levels in SubtiExpress: yxjG | |
| Metabolic function and regulation of this protein in SubtiPathways: yxjG | |
| MW, pI | 42 kDa, 5.005 |
| Gene length, protein length | 1134 bp, 378 aa |
| Immediate neighbours | yxjH, yxjF |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU38960
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): YxjH
Extended information on the protein
- Kinetic information:
- Modification:
- S-bacillithiolation upon hypochlorite stress on Cys-346 PubMed
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 1YPX (the enzyme from Listeria monocytogenes, 49% identity)
- UniProt: P42318
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: yxjG PubMed
- Sigma factor:
- Regulatory mechanism:
- S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Bui Khanh Chi, Katrin Gronau, Ulrike Mäder, Bernd Hessling, Dörte Becher, Haike Antelmann
S-bacillithiolation protects against hypochlorite stress in Bacillus subtilis as revealed by transcriptomics and redox proteomics.
Mol Cell Proteomics: 2011, 10(11);M111.009506
[PubMed:21749987]
[WorldCat.org]
[DOI]
(I p)
Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532]
[WorldCat.org]
[DOI]
(I p)
Jerneja Tomsic, Brooke A McDaniel, Frank J Grundy, Tina M Henkin
Natural variability in S-adenosylmethionine (SAM)-dependent riboswitches: S-box elements in bacillus subtilis exhibit differential sensitivity to SAM In vivo and in vitro.
J Bacteriol: 2008, 190(3);823-33
[PubMed:18039762]
[WorldCat.org]
[DOI]
(I p)
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147]
[WorldCat.org]
[DOI]
(P p)
F J Grundy, T M Henkin
The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria.
Mol Microbiol: 1998, 30(4);737-49
[PubMed:10094622]
[WorldCat.org]
[DOI]
(P p)
