Difference between revisions of "Kbl"
| Line 54: | Line 54: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17000&redirect=T BSU17000] | ||
* '''DBTBS entry:''' no entry | * '''DBTBS entry:''' no entry | ||
| Line 92: | Line 93: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17000&redirect=T BSU17000] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1FC4 1FC4] (from ''Escherichia coli'', 40% identity, 60% similarity) {{PubMed|11318637}} | * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1FC4 1FC4] (from ''Escherichia coli'', 40% identity, 60% similarity) {{PubMed|11318637}} | ||
Revision as of 13:47, 2 April 2014
- Description: 2-amino-3-ketobutyrate CoA ligase
| Gene name | kbl |
| Synonyms | |
| Essential | no |
| Product | 2-amino-3-ketobutyrate CoA ligase |
| Function | threonine utilization |
| Gene expression levels in SubtiExpress: kbl | |
| Metabolic function and regulation of this protein in SubtiPathways: kbl | |
| MW, pI | 43 kDa, 6.25 |
| Gene length, protein length | 1176 bp, 392 aa |
| Immediate neighbours | tdh, ymcB |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU17000
Phenotypes of a mutant
Database entries
- BsubCyc: BSU17000
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Acetyl-CoA + glycine = CoA + 2-amino-3-oxobutanoate (according to Swiss-Prot)
- Protein family: class-II pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- pyridoxal-phosphate (according to Swiss-Prot)
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU17000
- UniProt: O31777
- KEGG entry: [2]
- E.C. number: 2.3.1.29
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Germán Plata, Tobias Fuhrer, Tzu-Lin Hsiao, Uwe Sauer, Dennis Vitkup
Global probabilistic annotation of metabolic networks enables enzyme discovery.
Nat Chem Biol: 2012, 8(10);848-54
[PubMed:22960854]
[WorldCat.org]
[DOI]
(I p)
A Schmidt, J Sivaraman, Y Li, R Larocque, J A Barbosa, C Smith, A Matte, J D Schrag, M Cygler
Three-dimensional structure of 2-amino-3-ketobutyrate CoA ligase from Escherichia coli complexed with a PLP-substrate intermediate: inferred reaction mechanism.
Biochemistry: 2001, 40(17);5151-60
[PubMed:11318637]
[WorldCat.org]
[DOI]
(P p)
