Difference between revisions of "PlsX"

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* '''Description:''' acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4) <br/><br/>
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* '''Description:''' acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4), coordinates membrane synthesis with [[cell division]] <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylpD ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylpD ''
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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|style="background:#ABCDEF;" align="center"| '''Essential''' || yes {{PubMed|24224907,12682299}}
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Product''' || acyl-acyl carrier protein (ACP):phosphate acyltransferase  
 
|style="background:#ABCDEF;" align="center"| '''Product''' || acyl-acyl carrier protein (ACP):phosphate acyltransferase  
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<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
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<br/><br/><br/><br/>
 
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<br/><br/><br/><br/><br/><br/>
 
  
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[cell division]]}},
 
{{SubtiWiki category|[[biosynthesis of lipids]]}},
 
{{SubtiWiki category|[[biosynthesis of lipids]]}},
 
{{SubtiWiki category|[[essential genes]]}}
 
{{SubtiWiki category|[[essential genes]]}}
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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** essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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** essential {{PubMed|24224907,12682299}}
 
** depletion results in sporulation deficiency (via [[SpoIIR]]) {{PubMed|22111992}}
 
** depletion results in sporulation deficiency (via [[SpoIIR]]) {{PubMed|22111992}}
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** a ts mutant forms an abberant Z-ring and has a [[cell division]] defect {{PubMed|24224907}}   
 +
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
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* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
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* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
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** [[PlsX]]-[[PlsY]] {{PubMed|19282621}}
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** [[PlsX]]-[[PlsY]] {{PubMed|19282621}}
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** [[PlsX]]-[[FtsA]] {{PubMed|24224907}}  
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
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** localizes to the cell division sites, colocalizes with the Z-ring {{PubMed|24224907}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=plsX_1662547_1663548_1 plsX] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=plsX_1662547_1663548_1 plsX] {{PubMed|22383849}}
  
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* '''Sigma factor:''' [[SigA]] {{PubMed|16932747}}
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* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16932747}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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=References=
 
=References=
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'''Additional publications:''' {{PubMed|22111992}}
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<pubmed>12737802, 19282621, 17645809, 17557823, 8759840 16932747 19850612 16091051 21383089 22111992 24224907</pubmed>
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<pubmed>12737802, 19282621, 17645809, 17557823, 8759840 16932747 19850612 16091051 21383089</pubmed>
 
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 19:18, 12 January 2014

  • Description: acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4), coordinates membrane synthesis with cell division

Gene name plsX
Synonyms ylpD
Essential yes PubMed
Product acyl-acyl carrier protein (ACP):phosphate acyltransferase
Function biosynthesis of phospholipids
Gene expression levels in SubtiExpress: plsX
Interactions involving this protein in SubtInteract: PlsX
Metabolic function and regulation of this protein in SubtiPathways:
plsX
MW, pI 35 kDa, 5.492
Gene length, protein length 999 bp, 333 aa
Immediate neighbours fapR, fabD
Sequences Protein DNA DNA_with_flanks
Genetic context
PlsX context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PlsX expression.png















Categories containing this gene/protein

cell division, biosynthesis of lipids, essential genes

This gene is a member of the following regulons

ComA regulon, FapR regulon

The gene

Basic information

  • Locus tag: BSU15890

Phenotypes of a mutant


Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)
  • Protein family: plsX family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced if the cells experience an accumulation of malonyl-CoA (FapR) PubMed
    • expressed at high cell density (ComA) PubMed
    • induced upon fatty acid biosynthesis inhibition PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Matsumoto lab PubMed
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hiraku Takada, Sanae Fukushima-Tanaka, Masato Morita, Yasuhiro Kasahara, Satoru Watanabe, Taku Chibazakura, Hiroshi Hara, Kouji Matsumoto, Hirofumi Yoshikawa
An essential enzyme for phospholipid synthesis associates with the Bacillus subtilis divisome.
Mol Microbiol: 2014, 91(2);242-55
[PubMed:24224907] [WorldCat.org] [DOI] (I p)

Veronica Diez, Gustavo E Schujman, Frederico J Gueiros-Filho, Diego de Mendoza
Vectorial signalling mechanism required for cell-cell communication during sporulation in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);261-74
[PubMed:22111992] [WorldCat.org] [DOI] (I p)

Michaela Wenzel, Malay Patra, Dirk Albrecht, David Y-K Chen, K C Nicolaou, Nils Metzler-Nolte, Julia E Bandow
Proteomic signature of fatty acid biosynthesis inhibition available for in vivo mechanism-of-action studies.
Antimicrob Agents Chemother: 2011, 55(6);2590-6
[PubMed:21383089] [WorldCat.org] [DOI] (I p)

Mariano A Martinez, Diego de Mendoza, Gustavo E Schujman
Transcriptional and functional characterization of the gene encoding acyl carrier protein in Bacillus subtilis.
Microbiology (Reading): 2010, 156(Pt 2);484-495
[PubMed:19850612] [WorldCat.org] [DOI] (I p)

Yoshinori Hara, Masahide Seki, Satoshi Matsuoka, Hiroshi Hara, Atsushi Yamashita, Kouji Matsumoto
Involvement of PlsX and the acyl-phosphate dependent sn-glycerol-3-phosphate acyltransferase PlsY in the initial stage of glycerolipid synthesis in Bacillus subtilis.
Genes Genet Syst: 2008, 83(6);433-42
[PubMed:19282621] [WorldCat.org] [DOI] (P p)

Mika Yoshimura, Taku Oshima, Naotake Ogasawara
Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli.
BMC Microbiol: 2007, 7;69
[PubMed:17645809] [WorldCat.org] [DOI] (I e)

Luciana Paoletti, Ying-Jie Lu, Gustavo E Schujman, Diego de Mendoza, Charles O Rock
Coupling of fatty acid and phospholipid synthesis in Bacillus subtilis.
J Bacteriol: 2007, 189(16);5816-24
[PubMed:17557823] [WorldCat.org] [DOI] (P p)

Gustavo E Schujman, Marcelo Guerin, Alejandro Buschiazzo, Francis Schaeffer, Leticia I Llarrull, Georgina Reh, Alejandro J Vila, Pedro M Alzari, Diego de Mendoza
Structural basis of lipid biosynthesis regulation in Gram-positive bacteria.
EMBO J: 2006, 25(17);4074-83
[PubMed:16932747] [WorldCat.org] [DOI] (P p)

Natalia Comella, Alan D Grossman
Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis.
Mol Microbiol: 2005, 57(4);1159-74
[PubMed:16091051] [WorldCat.org] [DOI] (P p)

Gustavo E Schujman, Luciana Paoletti, Alan D Grossman, Diego de Mendoza
FapR, a bacterial transcription factor involved in global regulation of membrane lipid biosynthesis.
Dev Cell: 2003, 4(5);663-72
[PubMed:12737802] [WorldCat.org] [DOI] (P p)

H R Morbidoni, D de Mendoza, J E Cronan
Bacillus subtilis acyl carrier protein is encoded in a cluster of lipid biosynthesis genes.
J Bacteriol: 1996, 178(16);4794-800
[PubMed:8759840] [WorldCat.org] [DOI] (P p)

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