FeuA

• Description: ABC transporter for the siderophores Fe-enterobactin and Fe-bacillibactin (binding protein), with YusV as ATPase
  
  

Gene name	feuA
Synonyms
Essential	no
Product	ABC transporter for the siderophores Fe-enterobactin and Fe-bacillibactin (binding protein)
Function	acquisition of iron
Gene expression levels in SubtiExpress: feuA
Interactions involving this protein in SubtInteract: FeuA
Metabolic function and regulation of this protein in SubtiPathways: feuA
MW, pI	34 kDa, 8.018
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	feuB, btr
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, acquisition of iron, iron metabolism, membrane proteins

This gene is a member of the following regulons

Btr regulon, CitB regulon, Fur regulon

The gene

Basic information

• Locus tag: BSU01630

Database entries

• BsubCyc: BSU01630

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/biological activity:

Substrate binding protein for the triscatecholate siderophores ferri-bacillibactin and ferri-enterobactin and part of the FeuA-FeuB-FeuC-YusV ATP-binding cassette-type transporter. Furthermore ferric complexes of L-norepinephrine are bound by this protein.

• Protein family: Fe/B12 periplasmic-binding domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

Two independent non-symmetric globular domains, connected by a long alpha-helix (residues 143-164 of the mature protein).

• Modification:
  • FeuA is a lipoprotein with a N-acetyl-S-diacyl-glyceryl-cysteine structure PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YusV-(FeuB-FeuC)-FeuA PubMed

• Localization:
  • associated to the membrane (lipoprotein) PubMed
  • extracellular (signal peptide) PubMed

Database entries

• BsubCyc: BSU01630

• Structure: 2PHZ, 2WI8, 2WHY (complex with ferri-bacillibactin), 2XUZ (complex with ferri-enterobactin), 2XV1 (complex with ferric mecam)

• UniProt: P40409

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: feuA-feuB-feuC-ybbA PubMed

• Expression browser: feuA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of iron (Fur, CitB) PubMed
  • induced in the presence of iron chelators bacillibactin or enterobactin (Btr) PubMed

• Regulatory mechanism:
  • Fur: transcription repression PubMed
  • CitB: binding to the iron responsive element in the absence of iron
  • Btr: transcription activation in the presence of the co-activators bacillibactin or enterobactin PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed