Ctc

• Description: general stress protein, similar to ribosomal protein L25
  
  

Gene name	ctc
Synonyms
Essential	no
Product	ribosomal protein
Function	unknown
Gene expression levels in SubtiExpress: ctc
Interactions involving this protein in SubtInteract: Ctc
Metabolic function and regulation of this protein in SubtiPathways: ctc
MW, pI	21 kDa, 4.216
Gene length, protein length	612 bp, 204 aa
Immediate neighbours	prs, spoVC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU00520

Database entries

• BsubCyc: BSU00520

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: Ctc subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • 5S rRNA-Ctc PubMed

• Localization: in the ribosome (large subunit) PubMed

Database entries

• BsubCyc: BSU00520

• Structure: 1FEU (from Thermus thermophilus, 32% identity, 53% similarity) PubMed

• UniProt: P14194

• KEGG entry: [3]

• E.C. number:

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon:
  • gcaD-prs-ctc PubMed
  • ctc PubMed

• Expression browser: ctc PubMed

• Sigma factor:
  • gcaD: SigA PubMed
  • ctc: SigB PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism: alternative sigma factor, SigB PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant: GP500(spc), available in Stülke lab

• Expression vector:
  • expression/ purification with N-terminal His-tag in E. coli: pGP804 (in pWH844), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Stülke lab

References

Reviews

Additional reviews: PubMed

Original publications

Genki Akanuma, Hideaki Nanamiya, Yousuke Natori, Koichi Yano, Shota Suzuki, Shuya Omata, Morio Ishizuka, Yasuhiko Sekine, Fujio Kawamura
Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation.
J Bacteriol: 2012, 194(22);6282-91
[PubMed:23002217] [WorldCat.org] [DOI] (I p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Matthias Schmalisch, Ines Langbein, Jörg Stülke
The general stress protein Ctc of Bacillus subtilis is a ribosomal protein.
J Mol Microbiol Biotechnol: 2002, 4(5);495-501
[PubMed:12432960] [WorldCat.org] (P p)

R Fedorov, V Meshcheryakov, G Gongadze, N Fomenkova, N Nevskaya, M Selmer, M Laurberg, O Kristensen, S Al-Karadaghi, A Liljas, M Garber, S Nikonov
Structure of ribosomal protein TL5 complexed with RNA provides new insights into the CTC family of stress proteins.
Acta Crystallogr D Biol Crystallogr: 2001, 57(Pt 7);968-76
[PubMed:11418764] [WorldCat.org] [DOI] (P p)

I Hilden, B N Krath, B Hove-Jensen
Tricistronic operon expression of the genes gcaD (tms), which encodes N-acetylglucosamine 1-phosphate uridyltransferase, prs, which encodes phosphoribosyl diphosphate synthetase, and ctc in vegetative cells of Bacillus subtilis.
J Bacteriol: 1995, 177(24);7280-4
[PubMed:8522540] [WorldCat.org] [DOI] (P p)

C L Truitt, E A Weaver, W G Haldenwang
Effects on growth and sporulation of inactivation of a Bacillus subtilis gene (ctc) transcribed in vitro by minor vegetative cell RNA polymerases (E-sigma 37, E-sigma 32).
Mol Gen Genet: 1988, 212(1);166-71
[PubMed:2836704] [WorldCat.org] [DOI] (P p)

C Ray, M Igo, W Shafer, R Losick, C P Moran
Suppression of ctc promoter mutations in Bacillus subtilis.
J Bacteriol: 1988, 170(2);900-7
[PubMed:3123466] [WorldCat.org] [DOI] (P p)

M M Igo, R Losick
Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis.
J Mol Biol: 1986, 191(4);615-24
[PubMed:3100810] [WorldCat.org] [DOI] (P p)

J F Ollington, W G Haldenwang, T V Huynh, R Losick
Developmentally regulated transcription in a cloned segment of the Bacillus subtilis chromosome.
J Bacteriol: 1981, 147(2);432-42
[PubMed:6790515] [WorldCat.org] [DOI] (P p)

W G Haldenwang, R Losick
A modified RNA polymerase transcribes a cloned gene under sporulation control in Bacillus subtilis.
Nature: 1979, 282(5736);256-60
[PubMed:116131] [WorldCat.org] [DOI] (P p)