CapB

• Description: capsular polyglutamate biosynthesis
  
  

Gene name	capB
Synonyms	ywsC, pgsB
Essential	no
Product	poly-gamma-glutamate synthetase
Function	capsule synthesis
Gene expression levels in SubtiExpress: capB
MW, pI	43 kDa, 5.073
Gene length, protein length	1179 bp, 393 aa
Immediate neighbours	capC, rbsR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

capsule biosynthesis and degradation

This gene is a member of the following regulons

DegU regulon

The gene

Basic information

• Locus tag: BSU35900

Phenotypes of a mutant

no synthesis of the poly-gamma-glutamate capsule PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: synthesis of poly-gamma-glutamate from L-Glu PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P96736

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: capB-capC-capA-capE PubMed

• Expression browser: capB PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • DegU-P: transcription activation PubMed

• Additional information:
  • The operon is not expressed in the lab strain 168 due to lack of expression of DegQ resulting in inactivity of DegS and DegU PubMed
  • expression of the operon is reduced in motA or motB mutants due to reduced DegU phosphorylation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)

Thomas Candela, Agnès Fouet
Poly-gamma-glutamate in bacteria.
Mol Microbiol: 2006, 60(5);1091-8
[PubMed:16689787] [WorldCat.org] [DOI] (P p)