SirA

• Description: sporulation protein, inhibits DNA replication
  
  

Gene name	sirA
Synonyms	yneE, yoxF
Essential	no
Product	inhibitor of DNA replication
Function	control of chromosome copy number
Gene expression levels in SubtiExpress: sirA
Interactions involving this protein in SubtInteract: SirA
MW, pI	17 kDa, 7.353
Gene length, protein length	444 bp, 148 aa
Immediate neighbours	tkt, yneF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication

This gene is a member of the following regulons

Spo0A regulon

The gene

yneE was renamed to sirA.

Basic information

• Locus tag: BSU17900

Phenotypes of a mutant

"over-replication" during sporulation PubMed

Database entries

• BsubCyc: BSU17900

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: interacts with DnaA and displaces it from the replication origin, resulting in inhibition of replication PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SirA-DnaA PubMed

• Localization:

Database entries

• BsubCyc: BSU17900

• Structure:

• UniProt: P45707

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sirA-yneF PubMed

• Expression browser: sirA PubMed

• Sigma factor:

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Your additional remarks

sirA (sporulation inhibitor of replication) is activated by Spo0A~P and inhibits DNA replication during sporulation.

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Tsutomu Katayama, Shogo Ozaki, Kenji Keyamura, Kazuyuki Fujimitsu
Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC.
Nat Rev Microbiol: 2010, 8(3);163-70
[PubMed:20157337] [WorldCat.org] [DOI] (I p)