TsaD

• Description: required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with TsaC), universally conserved protein
  
  

Gene name	tsaD
Synonyms	ydiE, gcp
Essential	yes PubMed
Product	tRNA modification enzyme
Function	tRNA modification
Gene expression levels in SubtiExpress: tsaD
Interactions involving this protein in SubtInteract: TsaD
MW, pI	36 kDa, 5.016
Gene length, protein length	1038 bp, 346 aa
Immediate neighbours	ydiD, ydiF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, universally conserved proteins

The gene

Basic information

• Locus tag: BSU05940

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU05940

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP (TsaB-TsaD-TsaE) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TsaB-TsaD PubMed
  • TsaD-TsaE PubMed

• Localization:

Database entries

• BsubCyc: BSU05940

• Structure:

• UniProt: O05518

• KEGG entry: [2]

• E.C. number:

Additional information

universally conserved protein

Expression and regulation

• Operon: thiL-tsaE-tsaB-ydiD-tsaD PubMed

• Expression browser: tsaD PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: