Difference between revisions of "ComX"
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * the mutation suppresses the mucoid phenotype of ''[[motA]]'' or ''[[motB]]'' mutants due to loss of [[DegU]] phosphorylation and concomitant reduced expression of the ''[[pgsB]]-[[pgsC]]-[[pgsA]]'' operon {{PubMed|24296669}} | ||
=== Database entries === | === Database entries === | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
| − | * '''Modification:''' is synthesized as an inactive precursor, cleaved, and modified (prenylated) by [[ComQ]] prior to excretion {{PubMed|22878193}} | + | * '''Modification:''' |
| + | ** is synthesized as an inactive precursor, cleaved, and modified (prenylated) by [[ComQ]] prior to excretion {{PubMed|22878193}} | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comX_3255853_3256020_-1 comX] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comX_3255853_3256020_-1 comX] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigA]] {{PubMed|1715859}} | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1715859}} |
* '''Regulation:''' | * '''Regulation:''' | ||
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<pubmed> 22024380 </pubmed> | <pubmed> 22024380 </pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | + | <pubmed>16407988,11133937,12067344,19202088,14679219,15564679,17240141,19605685, 1715859 22878193 24296669 22511326,21636272 </pubmed> | |
| − | <pubmed>16407988,11133937,12067344,19202088,14679219,15564679,17240141,19605685, 1715859 22878193</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 20:06, 4 December 2013
- Description: competence pheromone precursor, transfers cell density signal to ComP; triggers the production of surfactin
| Gene name | comX |
| Synonyms | |
| Essential | no |
| Product | competence pheromone precursor |
| Function | quorum sensing |
| Gene expression levels in SubtiExpress: comX | |
| Interactions involving this protein in SubtInteract: ComX | |
| MW, pI | 6 kDa, 4.358 |
| Gene length, protein length | 165 bp, 55 aa |
| Immediate neighbours | comP, comQ |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
genetic competence, quorum sensing
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31700
Phenotypes of a mutant
- the mutation suppresses the mucoid phenotype of motA or motB mutants due to loss of DegU phosphorylation and concomitant reduced expression of the pgsB-pgsC-pgsA operon PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization:
- secreted (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P45453
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
The ComX --> surfactin signaling pathway is an example for paracrine signaling in bacteria
References
Reviews
Original publications
Jia Mun Chan, Sarah B Guttenplan, Daniel B Kearns
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
J Bacteriol: 2014, 196(4);740-53
[PubMed:24296669]
[WorldCat.org]
[DOI]
(I p)
Fumitada Tsuji, Ayako Ishihara, Aya Nakagawa, Masahiro Okada, Shigeyuki Kitamura, Kyoko Kanamaru, Yuichi Masuda, Kazuma Murakami, Kazuhiro Irie, Youji Sakagami
Lack of the consensus sequence necessary for tryptophan prenylation in the ComX pheromone precursor.
Biosci Biotechnol Biochem: 2012, 76(8);1492-6
[PubMed:22878193]
[WorldCat.org]
[DOI]
(I p)
Ju Jung, Kyung Ok Yu, Ahmad Bazli Ramzi, Se Hoon Choe, Seung Wook Kim, Sung Ok Han
Improvement of surfactin production in Bacillus subtilis using synthetic wastewater by overexpression of specific extracellular signaling peptides, comX and phrC.
Biotechnol Bioeng: 2012, 109(9);2349-56
[PubMed:22511326]
[WorldCat.org]
[DOI]
(I p)
Fumitada Tsuji, Ko Kobayashi, Masahiro Okada, Hisao Yamaguchi, Makoto Ojika, Youji Sakagami
The geranyl-modified tryptophan residue is crucial for ComXRO-E-2 pheromone biological activity.
Bioorg Med Chem Lett: 2011, 21(13);4041-4
[PubMed:21636272]
[WorldCat.org]
[DOI]
(I p)
Daniel López, Hera Vlamakis, Richard Losick, Roberto Kolter
Paracrine signaling in a bacterium.
Genes Dev: 2009, 23(14);1631-8
[PubMed:19605685]
[WorldCat.org]
[DOI]
(I p)
Kassem Hamze, Daria Julkowska, Sabine Autret, Krzysztof Hinc, Krzysztofa Nagorska, Agnieszka Sekowska, I Barry Holland, Simone J Séror
Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC.
Microbiology (Reading): 2009, 155(Pt 2);398-412
[PubMed:19202088]
[WorldCat.org]
[DOI]
(P p)
Masahiro Okada, Hisao Yamaguchi, Isao Sato, Soo Jeong Cho, David Dubnau, Youji Sakagami
Structure-activity relationship studies on quorum sensing ComX(RO-E-2) pheromone.
Bioorg Med Chem Lett: 2007, 17(6);1705-7
[PubMed:17240141]
[WorldCat.org]
[DOI]
(P p)
Masahiro Okada, Isao Sato, Soo Jeong Cho, Hidehisa Iwata, Toshihiko Nishio, David Dubnau, Youji Sakagami
Structure of the Bacillus subtilis quorum-sensing peptide pheromone ComX.
Nat Chem Biol: 2005, 1(1);23-4
[PubMed:16407988]
[WorldCat.org]
[DOI]
(P p)
Masahiro Okada, Isao Sato, Soo Jeong Cho, Yoshihiro Suzuki, Makoto Ojika, David Dubnau, Youji Sakagami
Towards structural determination of the ComX pheromone: synthetic studies on peptides containing geranyltryptophan.
Biosci Biotechnol Biochem: 2004, 68(11);2374-87
[PubMed:15564679]
[WorldCat.org]
[DOI]
(P p)
M Ansaldi, D Dubnau
Diversifying selection at the Bacillus quorum-sensing locus and determinants of modification specificity during synthesis of the ComX pheromone.
J Bacteriol: 2004, 186(1);15-21
[PubMed:14679219]
[WorldCat.org]
[DOI]
(P p)
Mireille Ansaldi, Darja Marolt, Tina Stebe, Ines Mandic-Mulec, David Dubnau
Specific activation of the Bacillus quorum-sensing systems by isoprenylated pheromone variants.
Mol Microbiol: 2002, 44(6);1561-73
[PubMed:12067344]
[WorldCat.org]
[DOI]
(P p)
P Tortosa, L Logsdon, B Kraigher, Y Itoh, I Mandic-Mulec, D Dubnau
Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system.
J Bacteriol: 2001, 183(2);451-60
[PubMed:11133937]
[WorldCat.org]
[DOI]
(P p)
Y Weinrauch, T Msadek, F Kunst, D Dubnau
Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis.
J Bacteriol: 1991, 173(18);5685-93
[PubMed:1715859]
[WorldCat.org]
[DOI]
(P p)
