RapA

• Description: response regulator aspartate phosphatase, dephosphorylates Spo0F-P, control of the phosphorelay
  
  

Gene name	rapA
Synonyms	gsiAA, spo0L
Essential	no
Product	response regulator aspartate phosphatase
Function	control of sporulation initiation
Gene expression levels in SubtiExpress: rapA
Interactions involving this protein in SubtInteract: RapA
Function and regulation of this protein in SubtiPathways: rapA
MW, pI	44 kDa, 4.848
Gene length, protein length	1134 bp, 378 aa
Immediate neighbours	yjoB, phrA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphorelay, quorum sensing

This gene is a member of the following regulons

CodY regulon, ComA regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU12430

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • modular organization comprising an amino terminal alpha-helical domain connected to a domain formed by six tetratricopeptide (TPR) repeats PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RapA (central TPR3-5 repeats) -PhrA PubMed
  • RapA (N-terminal 60 amino acids) -Spo0F PubMed

• Localization:

Database entries

• Structure:

• UniProt: Q00828

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapA-phrA PubMed

• Expression browser: rapA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (4.2-fold) (CcpA) PubMed
  • repressed by casamino acids PubMed
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • ComA: transcription activation (ComA) PubMed
  • CcpA: transcription repression PubMed
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: