YqhY

• Description: unknown
  
  

Gene name	yqhY
Synonyms
Essential	yes PubMed
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: yqhY
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	14 kDa, 4.414
Gene length, protein length	405 bp, 135 aa
Immediate neighbours	nusB, accC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteins of unknown function, essential genes

The gene

Basic information

• Locus tag: BSU24330

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: asp23 family (according to Swiss-Prot)

• Paralogous protein(s): YloU

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54519

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: accB-accC-yqhY PubMed

• Expression browser: yqhY PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP1468 (yqhY::erm), available in Jörg Stülke's lab

• Expression vector:
  • GP1474 (chromosomal yqhY-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
  • pGP1322 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
  • pGP1325 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:
  • pBP43-yqhY-Gfp, available in Jörg Stülke's lab

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• FLAG-tag construct:
  • GP1476 (spc aphA3, based on pGP1331), available in Jörg Stülke's lab

• Antibody:

References

Fabian M Commichau, Nico Pietack, Jörg Stülke
Essential genes in Bacillus subtilis: a re-evaluation after ten years.
Mol Biosyst: 2013, 9(6);1068-75
[PubMed:23420519] [WorldCat.org] [DOI] (I p)

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)

P Marini, S J Li, D Gardiol, J E Cronan, D de Mendoza
The genes encoding the biotin carboxyl carrier protein and biotin carboxylase subunits of Bacillus subtilis acetyl coenzyme A carboxylase, the first enzyme of fatty acid synthesis.
J Bacteriol: 1995, 177(23);7003-6
[PubMed:7592499] [WorldCat.org] [DOI] (P p)