AraE

• Description: L-arabinose permease
  
  

Gene name	araE
Synonyms	yvbR
Essential	no
Product	L-arabinose permease
Function	uptake of arabinose, galactose and xylose
Gene expression levels in SubtiExpress: araE
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	50 kDa, 9.259
Gene length, protein length	1392 bp, 464 aa
Immediate neighbours	cggR, araR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

AraR regulon, CcpA regulon

The gene

Basic information

• Locus tag: BSU33960

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transports arabinose, and also xylose and galactose PubMed

• Protein family:

• Paralogous protein(s): CsbC, IolT, YfiG, YncC, YwtG

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:
  • 4JA4 (the xylose transporter XylE from E. coli, 39% identity, 68% similarity) PubMed

• UniProt: P96710

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: araE PubMed

• Expression browser: araE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by arabinose (AraR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • AraR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Esben M Quistgaard, Christian Löw, Per Moberg, Lionel Trésaugues, Pär Nordlund
Structural basis for substrate transport in the GLUT-homology family of monosaccharide transporters.
Nat Struct Mol Biol: 2013, 20(6);766-8
[PubMed:23624861] [WorldCat.org] [DOI] (I p)

José Manuel Inácio, Carla Costa, Isabel de Sá-Nogueira
Distinct molecular mechanisms involved in carbon catabolite repression of the arabinose regulon in Bacillus subtilis.
Microbiology (Reading): 2003, 149(Pt 9);2345-2355
[PubMed:12949161] [WorldCat.org] [DOI] (P p)

L J Mota, P Tavares, I Sá-Nogueira
Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis.
Mol Microbiol: 1999, 33(3);476-89
[PubMed:10417639] [WorldCat.org] [DOI] (P p)

O Krispin, R Allmansberger
The Bacillus subtilis AraE protein displays a broad substrate specificity for several different sugars.
J Bacteriol: 1998, 180(12);3250-2
[PubMed:9620981] [WorldCat.org] [DOI] (P p)

I Sá-Nogueira, S S Ramos
Cloning, functional analysis, and transcriptional regulation of the Bacillus subtilis araE gene involved in L-arabinose utilization.
J Bacteriol: 1997, 179(24);7705-11
[PubMed:9401028] [WorldCat.org] [DOI] (P p)