Difference between revisions of "YdaO"
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* '''Mutant:''' | * '''Mutant:''' | ||
| − | ** 1A688 ( ''ydaO''::''erm''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC] | + | ** 1A688 (''ydaO''::''erm''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC] |
** GP93 (D''[[ydaO]]''::''cat''), available in [[Jörg Stülke]]'s lab | ** GP93 (D''[[ydaO]]''::''cat''), available in [[Jörg Stülke]]'s lab | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | * '''lacZ fusion:''' | + | * '''lacZ fusion: available in [[Fabian Commichau]]'s lab''' |
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
Revision as of 20:58, 1 August 2013
- Description: unknown
| Gene name | ydaO |
| Synonyms | |
| Essential | no |
| Product | unknown |
| Function | unknown |
| Gene expression levels in SubtiExpress: ydaO | |
| MW, pI | 66 kDa, 9.929 |
| Gene length, protein length | 1821 bp, 607 aa |
| Immediate neighbours | ydaN, mutT |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
membrane proteins, proteins of unknown function
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04320
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to UniProt)
Database entries
- Structure:
- UniProt: P96589
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- induced if the energy charge is low (ydaO riboswitch) PubMed
- Regulatory mechanism:
- expression is controlled via termination antitermination by the ydaO riboswitch PubMed
- Additional information:
Biological materials
- Mutant:
- 1A688 (ydaO::erm), PubMed, available at BGSC
- GP93 (DydaO::cat), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion: available in Fabian Commichau's lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Peter Y Watson, Martha J Fedor
The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis.
Nat Chem Biol: 2012, 8(12);963-5
[PubMed:23086297]
[WorldCat.org]
[DOI]
(I p)
Kirsten F Block, Ming C Hammond, Ronald R Breaker
Evidence for widespread gene control function by the ydaO riboswitch candidate.
J Bacteriol: 2010, 192(15);3983-9
[PubMed:20511502]
[WorldCat.org]
[DOI]
(I p)
Jeffrey E Barrick, Keith A Corbino, Wade C Winkler, Ali Nahvi, Maumita Mandal, Jennifer Collins, Mark Lee, Adam Roth, Narasimhan Sudarsan, Inbal Jona, J Kenneth Wickiser, Ronald R Breaker
New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control.
Proc Natl Acad Sci U S A: 2004, 101(17);6421-6
[PubMed:15096624]
[WorldCat.org]
[DOI]
(P p)
