Difference between revisions of "Hag"

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(Database entries)
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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId= 3A5X 3A5X] (from ''Salmonella typhimurium'', 42% identity) {{PubMed|23804755}}
+
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3A5X 3A5X] (from ''Salmonella typhimurium'', 42% identity) {{PubMed|23804755}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P02968 P02968]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P02968 P02968]

Revision as of 16:09, 29 July 2013

  • Description: flagellin protein, about 20,000 subunits make up one flagellum

Gene name hag
Synonyms
Essential no
Product flagellin protein
Function motility and chemotaxis
Gene expression levels in SubtiExpress: hag
Interactions involving this protein in SubtInteract: Hag
MW, pI 32 kDa, 4.782
Gene length, protein length 912 bp, 304 aa
Immediate neighbours yvyC, csrA
Sequences Protein DNA DNA_with_flanks
Genetic context
Hag context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Hag expression.png















Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, CsrA regulon, ScoC regulon, SigD regulon

The gene

Basic information

  • Locus tag: BSU35360

Phenotypes of a mutant

No swarming motility on B medium. PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: bacterial flagellin family (according to Swiss-Prot)
  • Paralogous protein(s): YvzB (C-terminal domain of Hag)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3A5X (from Salmonella typhimurium, 42% identity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:
    • the hag gene is strongly overexpressed in a ymdB mutant (loss of bistable gene expression) PubMed

Biological materials

  • Mutant: GP901 (aphA3), GP902 (tet), both available in Stülke lab
  • Expression vector:
    • expression in B. subtilis, in pBQ200: pGP1089, available in Stülke lab
  • lacZ fusion: pGP1035 (in pAC6), pGP755 (in pAC7), there is also a series of promoter deletion variants in pAC6 and pAC7, all available in Stülke lab
  • GFP fusion: BP494 (bglS:: (hag-promoter-cfp-aphA3)), BP496 (amyE:: (hag-promoter-iyfp-cat)), available in Stülke lab
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


Original Publications