Difference between revisions of "MstX"
| Line 35: | Line 35: | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
{{SubtiWiki category|[[protein secretion]]}}, | {{SubtiWiki category|[[protein secretion]]}}, | ||
| + | {{SubtiWiki category|[[biofilm formation]]}}, | ||
{{SubtiWiki category|[[membrane proteins]]}} | {{SubtiWiki category|[[membrane proteins]]}} | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| − | + | {{SubtiWiki regulon|[[SinR regulon]]}} | |
=The gene= | =The gene= | ||
| Line 47: | Line 48: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * reduced [[biofilm formation]] (can be suppressed by inactivation of ''[[sinR]]'') {{PubMed|23737939}} | ||
| + | * overexpression result in increased [[biofilm formation]] even in a domesticated strain background {{PubMed|23737939}} | ||
=== Database entries === | === Database entries === | ||
Revision as of 12:12, 6 June 2013
- Description: membrane-integrating protein for membrane protein expression, MISTIC
| Gene name | mstX |
| Synonyms | |
| Essential | no |
| Product | MISTIC |
| Function | unknown |
| Gene expression levels in SubtiExpress: mstX | |
| MW, pI | 13 kDa, 4.549 |
| Gene length, protein length | 330 bp, 110 aa |
| Immediate neighbours | yuzI, yugO |
| Gene sequence (+200bp) | Protein sequence |
| Genetic context File:MstX context.gif | |
Contents
Categories containing this gene/protein
protein secretion, biofilm formation, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31321
Phenotypes of a mutant
- reduced biofilm formation (can be suppressed by inactivation of sinR) PubMed
- overexpression result in increased biofilm formation even in a domesticated strain background PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: no entry
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery PubMed
Database entries
- UniProt: Q5BU39
- KEGG entry: [1]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Matthew E Lundberg, Eric C Becker, Senyon Choe
MstX and a putative potassium channel facilitate biofilm formation in Bacillus subtilis.
PLoS One: 2013, 8(5);e60993
[PubMed:23737939]
[WorldCat.org]
[DOI]
(I e)
Dilip K Debnath, Rajiv Vaid Basaiawmoit, Kåre Lehmann Nielsen, Daniel E Otzen
The role of membrane properties in Mistic folding and dimerisation.
Protein Eng Des Sel: 2011, 24(1-2);89-97
[PubMed:21097953]
[WorldCat.org]
[DOI]
(I p)
Hay Dvir, Matthew E Lundberg, Samir K Maji, Roland Riek, Senyon Choe
Mistic: cellular localization, solution behavior, polymerization, and fibril formation.
Protein Sci: 2009, 18(7);1564-70
[PubMed:19475664]
[WorldCat.org]
[DOI]
(I p)
Tarmo P Roosild, Mark Vega, Samantha Castronovo, Senyon Choe
Characterization of the family of Mistic homologues.
BMC Struct Biol: 2006, 6;10
[PubMed:16704729]
[WorldCat.org]
[DOI]
(I e)
Tarmo P Roosild, Jason Greenwald, Mark Vega, Samantha Castronovo, Roland Riek, Senyon Choe
NMR structure of Mistic, a membrane-integrating protein for membrane protein expression.
Science: 2005, 307(5713);1317-21
[PubMed:15731457]
[WorldCat.org]
[DOI]
(I p)
