Difference between revisions of "DnaC"
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=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2R6A 2R6A] (Geobacillus stearothermophilus), [http://www.rcsb.org/pdb/explore.do?structureId=2R6C 2R6C], [http://www.rcsb.org/pdb/explore.do?structureId=2R6D 2R6D], [http://www.rcsb.org/pdb/explore.do?structureId=2R6E 2R6E] | + | * '''Structure:''' |
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=2R6A 2R6A] (Geobacillus stearothermophilus), [http://www.rcsb.org/pdb/explore.do?structureId=2R6C 2R6C], [http://www.rcsb.org/pdb/explore.do?structureId=2R6D 2R6D], [http://www.rcsb.org/pdb/explore.do?structureId=2R6E 2R6E] | ||
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=4M4W 4M4W] (the [[DnaC]]-[[DnaI]]-[[DnaG]] complex) {{PubMed|24048025}} | ||
* '''UniProt:''' [http://www.uniprot.org/uniprot/P37469 P37469] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P37469 P37469] | ||
Revision as of 08:30, 23 September 2013
- Description: replicative DNA helicase, part of the replisome
| Gene name | dnaC |
| Synonyms | |
| Essential | yes PubMed |
| Product | replicative DNA helicase |
| Function | DNA replication |
| Gene expression levels in SubtiExpress: dnaC | |
| Interactions involving this protein in SubtInteract: DnaC | |
| MW, pI | 50 kDa, 4.548 |
| Gene length, protein length | 1362 bp, 454 aa |
| Immediate neighbours | yycE, yycD |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA replication, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU40440
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Protein family: DnaB subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: Cytoplasm (Homogeneous) PubMed
Database entries
- Structure:
- UniProt: P37469
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: dnaC (according to DBTBS)
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Geoffrey S Briggs, Wiep Klaas Smits, Panos Soultanas
Chromosomal replication initiation machinery of low-G+C-content Firmicutes.
J Bacteriol: 2012, 194(19);5162-70
[PubMed:22797751]
[WorldCat.org]
[DOI]
(I p)
Original publications
Olivier Rannou, Emmanuelle Le Chatelier, Marilynn A Larson, Hamid Nouri, Bérengère Dalmais, Charles Laughton, Laurent Jannière, Panos Soultanas
Functional interplay of DnaE polymerase, DnaG primase and DnaC helicase within a ternary complex, and primase to polymerase hand-off during lagging strand DNA replication in Bacillus subtilis.
Nucleic Acids Res: 2013, 41(10);5303-20
[PubMed:23563155]
[WorldCat.org]
[DOI]
(I p)
José P Afonso, Kiran Chintakayala, Chatrudee Suwannachart, Svetlana Sedelnikova, Kevin Giles, John B Hoyes, Panos Soultanas, John B Rafferty, Neil J Oldham
Insights into the structure and assembly of the Bacillus subtilis clamp-loader complex and its interaction with the replicative helicase.
Nucleic Acids Res: 2013, 41(9);5115-26
[PubMed:23525462]
[WorldCat.org]
[DOI]
(I p)
Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408]
[WorldCat.org]
[DOI]
(I p)
Wiep Klaas Smits, Alexi I Goranov, Alan D Grossman
Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo.
Mol Microbiol: 2010, 75(2);452-61
[PubMed:19968790]
[WorldCat.org]
[DOI]
(I p)
Kuang-Lei Tsai, Yu-Hua Lo, Yuh-Ju Sun, Chwan-Deng Hsiao
Molecular interplay between the replicative helicase DnaC and its loader protein DnaI from Geobacillus kaustophilus.
J Mol Biol: 2009, 393(5);1056-69
[PubMed:19744498]
[WorldCat.org]
[DOI]
(I p)
Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537]
[WorldCat.org]
[DOI]
(P p)
Marion Velten, Stephen McGovern, Stéphanie Marsin, S Dusko Ehrlich, Philippe Noirot, Patrice Polard
A two-protein strategy for the functional loading of a cellular replicative DNA helicase.
Mol Cell: 2003, 11(4);1009-20
[PubMed:12718886]
[WorldCat.org]
[DOI]
(P p)
Marie-Françoise Noirot-Gros, Etienne Dervyn, Ling Juan Wu, Peggy Mervelet, Jeffery Errington, S Dusko Ehrlich, Philippe Noirot
An expanded view of bacterial DNA replication.
Proc Natl Acad Sci U S A: 2002, 99(12);8342-7
[PubMed:12060778]
[WorldCat.org]
[DOI]
(P p)
M Bárcena, T Ruiz, L E Donate, S E Brown, N E Dixon, M Radermacher, J M Carazo
The DnaB.DnaC complex: a structure based on dimers assembled around an occluded channel.
EMBO J: 2001, 20(6);1462-8
[PubMed:11250911]
[WorldCat.org]
[DOI]
(P p)
Y Imai, N Ogasawara, D Ishigo-Oka, R Kadoya, T Daito, S Moriya
Subcellular localization of Dna-initiation proteins of Bacillus subtilis: evidence that chromosome replication begins at either edge of the nucleoids.
Mol Microbiol: 2000, 36(5);1037-48
[PubMed:10844689]
[WorldCat.org]
[DOI]
(P p)
