SacA

• Description: phosphosucrase
  
  

Gene name	sacA
Synonyms	ipa-50d
Essential	no
Product	phosphosucrase
Function	utilization of sucrose
Gene expression levels in SubtiExpress: sacA
Metabolic function and regulation of this protein in SubtiPathways: sacA
MW, pI	54 kDa, 5.34
Gene length, protein length	1440 bp, 480 aa
Immediate neighbours	ywdA, sacP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, SacT regulon

The gene

Basic information

• Locus tag: BSU38040

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P07819

• KEGG entry: [3]

• E.C. number: 3.2.1.26

Expression and regulation

• Operon: sacP-sacA-ywdA PubMed

• Expression browser: sacA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by sucrose (SacT) PubMed
  • repressed by glucose (CcpA)

• Regulatory mechanism:
  • SacT: binding to a RNA switch results in transcription antitermination PubMed
  • CcpA: transcription repression

• Additional information:

Biological materials

• Mutant:
  • 1A810 ( sacA::cat), PubMed, available at BGSC
  • 1A811 ( sacA::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

M Arnaud, M Débarbouillé, G Rapoport, M H Saier, J Reizer
In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis.
J Biol Chem: 1996, 271(31);18966-72
[PubMed:8702561] [WorldCat.org] [DOI] (P p)

M Debarbouille, M Arnaud, A Fouet, A Klier, G Rapoport
The sacT gene regulating the sacPA operon in Bacillus subtilis shares strong homology with transcriptional antiterminators.
J Bacteriol: 1990, 172(7);3966-73
[PubMed:2163394] [WorldCat.org] [DOI] (P p)

A Fouet, A Klier, G Rapoport
Nucleotide sequence of the sucrase gene of Bacillus subtilis.
Gene: 1986, 45(2);221-5
[PubMed:3100393] [WorldCat.org] [DOI] (P p)