Difference between revisions of "CotB"
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{{SubtiWiki regulon|[[GerE regulon]]}}, | {{SubtiWiki regulon|[[GerE regulon]]}}, | ||
{{SubtiWiki regulon|[[GerR regulon]]}}, | {{SubtiWiki regulon|[[GerR regulon]]}}, | ||
| − | {{SubtiWiki regulon|[[SigK regulon]]}} | + | {{SubtiWiki regulon|[[SigK regulon]]}}, |
| + | {{SubtiWiki regulon|[[SigE regulon]]}} | ||
=The gene= | =The gene= | ||
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* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| − | ** outer spore coat, at the middle of the spore as a ring- or spiral-like structure {{PubMed|19933362}} | + | ** outer spore coat, at the middle of the spore as a ring- or spiral-like structure, localization depends on [[CotE]] {{PubMed|22171814,19933362}} |
=== Database entries === | === Database entries === | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cotB_3714739_3715881_-1 cotB] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cotB_3714739_3715881_-1 cotB] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigK]] {{PubMed|15699190,1691789}} | + | * '''Sigma factor:''' [[SigE]] {{PubMed|15383836}}, [[SigK]] {{PubMed|15699190,1691789}}, |
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** expressed late during sporulation in the mother cell ([[SigK]], [[GerE]], [[GerR]]) {{PubMed|15699190,1691789,20435725}} | + | ** expressed late during sporulation in the mother cell ([[SigE]], [[SigK]], [[GerE]], [[GerR]]) {{PubMed|15699190,1691789,15383836,20435725}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
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=References= | =References= | ||
| − | <pubmed> 19933362,1518043,2821284,14762006,1691789, 19933362 15699190 20435725</pubmed> | + | <pubmed>22171814, 19933362,1518043,2821284,14762006,1691789, 19933362 15699190 20435725 15383836</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:27, 13 August 2013
- Description: spore coat protein (outer)
| Gene name | cotB |
| Synonyms | |
| Essential | no |
| Product | spore coat protein (outer) |
| Function | resistance of the spore |
| Gene expression levels in SubtiExpress: cotB | |
| Interactions involving this protein in SubtInteract: CotB | |
| MW, pI | 42 kDa, 9.831 |
| Gene length, protein length | 1140 bp, 380 aa |
| Immediate neighbours | ywrJ, cotH |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
GerE regulon, GerR regulon, SigK regulon, SigE regulon
The gene
Basic information
- Locus tag: BSU36050
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P07789
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814]
[WorldCat.org]
[DOI]
(I p)
Giuseppina Cangiano, Antonio Mazzone, Loredana Baccigalupi, Rachele Isticato, Patrick Eichenberger, Maurilio De Felice, Ezio Ricca
Direct and indirect control of late sporulation genes by GerR of Bacillus subtilis.
J Bacteriol: 2010, 192(13);3406-13
[PubMed:20435725]
[WorldCat.org]
[DOI]
(I p)
Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Localization of proteins to different layers and regions of Bacillus subtilis spore coats.
J Bacteriol: 2010, 192(2);518-24
[PubMed:19933362]
[WorldCat.org]
[DOI]
(I p)
Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190]
[WorldCat.org]
[DOI]
(P p)
Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836]
[WorldCat.org]
[DOI]
(I p)
Rita Zilhão, Mónica Serrano, Rachele Isticato, Ezio Ricca, Charles P Moran, Adriano O Henriques
Interactions among CotB, CotG, and CotH during assembly of the Bacillus subtilis spore coat.
J Bacteriol: 2004, 186(4);1110-9
[PubMed:14762006]
[WorldCat.org]
[DOI]
(P p)
L Zheng, R Halberg, S Roels, H Ichikawa, L Kroos, R Losick
Sporulation regulatory protein GerE from Bacillus subtilis binds to and can activate or repress transcription from promoters for mother-cell-specific genes.
J Mol Biol: 1992, 226(4);1037-50
[PubMed:1518043]
[WorldCat.org]
[DOI]
(P p)
L B Zheng, R Losick
Cascade regulation of spore coat gene expression in Bacillus subtilis.
J Mol Biol: 1990, 212(4);645-60
[PubMed:1691789]
[WorldCat.org]
[DOI]
(P p)
W Donovan, L B Zheng, K Sandman, R Losick
Genes encoding spore coat polypeptides from Bacillus subtilis.
J Mol Biol: 1987, 196(1);1-10
[PubMed:2821284]
[WorldCat.org]
[DOI]
(P p)
