CggR

• Description: repressor of the glycolytic gapA operon, DeoR family
  
  

Gene name	cggR
Synonyms	yvbQ
Essential	no
Product	central glycolytic genes regulator
Function	transcriptional regulator
Gene expression levels in SubtiExpress: cggR
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	37,2 kDa,5.68
Gene length, protein length	1020 bp, 340 amino acids
Immediate neighbours	gapA, araE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, transcription factors and their control, regulators of core metabolism

This gene is a member of the following regulons

CggR regulon

The CggR regulon:

• cggR-gapA-pgk-tpi-pgm-eno

The gene

Basic information

• Locus tag: BSU33950

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription repression of the glycolytic gapA operon

• Protein family: sorC transcriptional regulatory family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • DNA binding domain (H-T-H motif) (37–56)

• Modification:

• Cofactor(s):

• Effectors of protein activity: fructose 1.6-bisphosphate PubMed and dihydroxyacetone phosphate, glucose-6-phosphate and fructose-6-phosphate PubMed act as inducer and result in release of CggR from the DNA

• Interactions:
  • active as dimer (according to PubMed)

• Localization: cytoplasma PubMed

Database entries

• Structure: 2OKG ( effector binding domain), 3BXH (in complex with fructose-6-phosphate), complex with Fructose-6-Phosphate NCBI, effector binding domain NCBI

• UniProt: O32253

• KEGG entry: [3]

Expression and regulation

• Operon:
  • cggR-gapA-pgk-tpi-pgm-eno PubMed
  • cggR-gapA PubMed

• Expression browser: cggR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expression activated by glucose (77-fold) (CggR) PubMed

• Regulatory mechanism:
  • cggR: transcription repression PubMed

• Database entries: DBTBS

• Additional information:
  • The primary mRNAs of the operon are highly unstable. The primary mRNA is subject to processing at the very end of the cggR open reading frame. This results in stable mature gapA and gapA-pgk-tpiA-pgm-eno mRNAs. PubMed The processing event requires the RNase Y PubMed.
  • The intracellular concentration of CggR is about 230 nM (according to PubMed).
  • The accumulation of the cggR-gapA mRNA is strongly dependent on the presence of the YkzW peptide, due to stabilization of the mRNA PubMed.
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

• Mutant:
  • GP311 (in frame deletion), available in Jörg Stülke's lab
  • SM-NB7 (cggR-spc), available in Anne Galinier's and Boris Görke's labs

• Expression vector: pGP705 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

• lacZ fusion: pGP504 (in pAC7), pGP509 (in pAC6), available in Jörg Stülke's lab

• GFP fusion:

• Antibody: available in Jörg Stülke's lab

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France