Difference between revisions of "Fni"
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| − | * '''Description:''' isopentenyl diphosphate isomerase <br/><br/> | + | * '''Description:''' isopentenyl diphosphate isomerase, last (8th) step in the MEP pathway of isoprenoid biosynthesis <br/><br/> |
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|''fni'' | |''fni'' | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ypgA'' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ypgA, idi'' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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|style="background:#ABCDEF;" align="center"| '''Product''' || isopentenyl diphosphate isomerase | |style="background:#ABCDEF;" align="center"| '''Product''' || isopentenyl diphosphate isomerase | ||
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || MEP pathway of isoprenoid biosynthesis |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22870 fni] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22870 fni] | ||
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=== Additional information=== | === Additional information=== | ||
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=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1P0N 1P0N] (complex with FMN) | + | * '''Structure:''' |
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=1P0N 1P0N] (complex with FMN) | ||
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=1P0K 1P0K] | ||
* '''UniProt:''' [http://www.uniprot.org/uniprot/P50740 P50740] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P50740 P50740] | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fni_2393602_2394651_-1 fni] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fni_2393602_2394651_-1 fni] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
Revision as of 08:23, 9 July 2013
- Description: isopentenyl diphosphate isomerase, last (8th) step in the MEP pathway of isoprenoid biosynthesis
| Gene name | fni |
| Synonyms | ypgA, idi |
| Essential | no |
| Product | isopentenyl diphosphate isomerase |
| Function | MEP pathway of isoprenoid biosynthesis |
| Gene expression levels in SubtiExpress: fni | |
| MW, pI | 22 kDa, 6.101 |
| Gene length, protein length | 636 bp, 212 aa |
| Immediate neighbours | ypzI, ypfD |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis of lipids, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22870
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Isopentenyl diphosphate = dimethylallyl diphosphate (according to Swiss-Prot)
- Protein family: IPP isomerase type 2 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- UniProt: P50740
- KEGG entry: [2]
- E.C. number: 5.3.3.2
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547]
[WorldCat.org]
[DOI]
(P p)
Motoki Takagi, Kazuhide Kaneda, Tomohiro Shimizu, Yoichi Hayakawa, Haruo Seto, Tomohisa Kuzuyama
Bacillus subtilis ypgA gene is fni, a nonessential gene encoding type 2 isopentenyl diphosphate isomerase.
Biosci Biotechnol Biochem: 2004, 68(1);132-7
[PubMed:14745175]
[WorldCat.org]
[DOI]
(P p)
Stefan Steinbacher, Johannes Kaiser, Stefan Gerhardt, Wolfgang Eisenreich, Robert Huber, Adelbert Bacher, Felix Rohdich
Crystal structure of the type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase from Bacillus subtilis.
J Mol Biol: 2003, 329(5);973-82
[PubMed:12798687]
[WorldCat.org]
[DOI]
(P p)
