Difference between revisions of "DprA"
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| − | * '''Description:''' conveys incoming ssDNA to [[RecA]] <br/><br/> | + | * '''Description:''' conveys incoming ssDNA to [[RecA]]; facilitates the displacement of both SSBs ([[SsbB]] and [[SsbA]]), increases [[RecA]] nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing <br/><br/> |
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | ** strongly impaired genetic recombination {{PubMed|23779106}} | ||
=== Database entries === | === Database entries === | ||
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
| + | ** binds ssDNA, promotes displacement of [[SsbA]] and [[SsbB]] from ssDNA {{PubMed|23779106}} | ||
** provides [[RecA]] access to ssDNA during chromosomal transformation (together with [[RecO]]) {{PubMed|22373918}} | ** provides [[RecA]] access to ssDNA during chromosomal transformation (together with [[RecO]]) {{PubMed|22373918}} | ||
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* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** forms dimers {{PubMed|23779106}} | ||
** [[DprA]]-[[RecA]] {{PubMed|17803906,17630974}} | ** [[DprA]]-[[RecA]] {{PubMed|17803906,17630974}} | ||
** [[DprA]]-[[DprA]] {{PubMed|17803906}} | ** [[DprA]]-[[DprA]] {{PubMed|17803906}} | ||
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=References= | =References= | ||
| − | + | <pubmed>17630974,17803906,12421306,18045469 11948146 ,22373918 23779106 23440217</pubmed> | |
| − | <pubmed>17630974,17803906,12421306,18045469 11948146 ,22373918 </pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:54, 21 June 2013
- Description: conveys incoming ssDNA to RecA; facilitates the displacement of both SSBs (SsbB and SsbA), increases RecA nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing
| Gene name | dprA |
| Synonyms | smf |
| Essential | no |
| Product | recombination mediator protein |
| Function | DNA repair/ recombination |
| Gene expression levels in SubtiExpress: dprA | |
| Interactions involving this protein in SubtInteract: DprA | |
| MW, pI | 32 kDa, 9.755 |
| Gene length, protein length | 891 bp, 297 aa |
| Immediate neighbours | sucD, topA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
DNA repair/ recombination, genetic competence
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16110
Phenotypes of a mutant
- strongly impaired genetic recombination PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: smf family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P39813
- KEGG entry: [3]
Additional information
Expression and regulation
- Operon: dprA (according to DBTBS)
- Sigma factor:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Peter Graumann, Freiburg University, Germany homepage
Your additional remarks
References
