Difference between revisions of "DefB"
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| − | * '''Description:''' | + | * '''Description:''' N-formylcysteine deformylase, required for the conversion of S-methyl-cysteine to cysteine<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || N-formylcysteine deformylase |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of S-methyl-cysteine |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14560 ykrB] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14560 ykrB] | ||
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[ | + | {{SubtiWiki category|[[sulfur metabolism]]}} |
| − | |||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * no growth with S-methyl cysteine {{PubMed|23944997}} | ||
=== Database entries === | === Database entries === | ||
| Line 67: | Line 67: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' N-acetylcystein --> cysteine + acetate {{PubMed|23944997}} |
* '''Protein family:''' polypeptide deformylase family (according to Swiss-Prot) | * '''Protein family:''' polypeptide deformylase family (according to Swiss-Prot) | ||
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* '''Operon:''' | * '''Operon:''' | ||
| − | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=defB_1526195_1526749_-1 | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=defB_1526195_1526749_-1 defB] {{PubMed|22383849}} |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
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=References= | =References= | ||
| − | <pubmed>11429456,, </pubmed> | + | <pubmed>11429456,23944997, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:47, 18 August 2013
- Description: N-formylcysteine deformylase, required for the conversion of S-methyl-cysteine to cysteine
| Gene name | ykrB |
| Synonyms | defB |
| Essential | no |
| Product | N-formylcysteine deformylase |
| Function | utilization of S-methyl-cysteine |
| Gene expression levels in SubtiExpress: ykrB | |
| MW, pI | 20 kDa, 5.456 |
| Gene length, protein length | 552 bp, 184 aa |
| Immediate neighbours | ykrA, ykzV |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU14560
Phenotypes of a mutant
- no growth with S-methyl cysteine PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: N-acetylcystein --> cysteine + acetate PubMed
- Protein family: polypeptide deformylase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 1LQY (complex with antibiotic actinonin, Geobacillus stearothermophilus)
- UniProt: Q45495
- KEGG entry: [2]
- E.C. number: 3.5.1.88
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Che-Man Chan, Antoine Danchin, Philippe Marlière, Agnieszka Sekowska
Paralogous metabolism: S-alkyl-cysteine degradation in Bacillus subtilis.
Environ Microbiol: 2014, 16(1);101-17
[PubMed:23944997]
[WorldCat.org]
[DOI]
(I p)
Michael Haas, Dieter Beyer, Reinhold Gahlmann, Christoph Freiberg
YkrB is the main peptide deformylase in Bacillus subtilis, a eubacterium containing two functional peptide deformylases.
Microbiology (Reading): 2001, 147(Pt 7);1783-1791
[PubMed:11429456]
[WorldCat.org]
[DOI]
(P p)
