HtpX

• Description: stress-responsive membrane protease
  
  

Gene name	htpX
Synonyms	ykrL
Essential	no
Product	membrane protease
Function	quality control of membrane proteins
Gene expression levels in SubtiExpress: htpX
MW, pI	32 kDa, 10.157
Gene length, protein length	894 bp, 298 aa
Immediate neighbours	ykrK, ktrD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, proteolysis

This gene is a member of the following regulons

Rok regulon, YkrK regulon,

The gene

Basic information

• Locus tag: BSU13490

Phenotypes of a mutant

• increased sensitivity to membrane protein overproduction PubMed
• increased sensitivity to dissipation of the membrane potential by the addition of valinomycin PubMed

Database entries

• BsubCyc: BSU13490

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase M48B family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU13490

• Structure:

• UniProt: O31657

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: htpX PubMed

• Expression browser: htpX PubMed

• Sigma factor:

• Regulation:
  • induced by membrane protein overproduction (YkrK) PubMed
  • induced by dissipation of membrane potential (ΔΨ) by addition of a sublethal concentration of valinomycin PubMed
  • expression is increased due to mutations in resE, resB, sdhC, and sdhA PubMed
  • induced by salt stress PubMed

• Regulatory mechanism:
  • YkrK: transcription repression PubMed
  • Rok: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: