Difference between revisions of "LigA"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU06620&redirect=T BSU06620]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pcrBA-ligA-yerH.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pcrBA-ligA-yerH.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU06620&redirect=T BSU06620]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1B04 1B04] (adenylation domain, Geobacillus stearothermophilus)
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1B04 1B04] (adenylation domain, Geobacillus stearothermophilus)

Revision as of 13:08, 2 April 2014

  • Description: DNA ligase (NAD-dependent)

Gene name ligA
Synonyms lig, yerG
Essential yes PubMed
Product DNA ligase (NAD-dependent)
Function replication
Gene expression levels in SubtiExpress: ligA
MW, pI 74 kDa, 4.708
Gene length, protein length 2004 bp, 668 aa
Immediate neighbours pcrA, yerH
Sequences Protein DNA DNA_with_flanks
Genetic context
LigA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LigA expression.png
























Categories containing this gene/protein

DNA replication, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

  • Locus tag: BSU06620

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m) (according to Swiss-Prot)
  • Protein family: BRCT domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1B04 (adenylation domain, Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References