PurL

• Description: phosphoribosylformylglycinamidine synthase
  
  

Gene name	purL
Synonyms
Essential	no
Product	phosphoribosylformylglycinamidine synthase
Function	purine biosynthesis
Gene expression levels in SubtiExpress: purL
Interactions involving this protein in SubtInteract: PurL
Metabolic function and regulation of this protein in SubtiPathways: Purine synthesis, Nucleotides (regulation)
MW, pI	80 kDa, 4.554
Gene length, protein length	2226 bp, 742 aa
Immediate neighbours	purQ, purF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, membrane proteins

This gene is a member of the following regulons

G-box, PurR regulon

The gene

Basic information

• Locus tag: BSU06480

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H₂O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate (according to Swiss-Prot)

• Protein family: FGAMS family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • PurS-PurQ-PurL PubMed

• Localization: cytoplasm (according to Swiss-Prot), membrane associated PubMed

Database entries

• Structure: 3D54 (from Thermotoga maritima, 39% identity, 58% similarity) PubMed

• UniProt: P12042

• KEGG entry: [3]

• E.C. number: 6.3.5.3

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD PubMed

• Expression browser: purL PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of purine nucleotides (PurR) PubMed
  • repressed in the presence of adenine or adenosine(PurR) PubMed
  • repressed in the presence of guanine(G-box) PubMed

• Regulatory mechanism:
  • [PurR]]: transcription repression PubMed
  • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • G-box: transcription termination/ antitermination (riboswitch) PubMed1 PubMed2

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed