Difference between revisions of "SacA"
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Revision as of 14:52, 16 May 2013
- Description: phosphosucrase
| Gene name | sacA |
| Synonyms | ipa-50d |
| Essential | no |
| Product | phosphosucrase |
| Function | utilization of sucrose |
| Gene expression levels in SubtiExpress: sacA | |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism | |
| MW, pI | 54 kDa, 5.34 |
| Gene length, protein length | 1440 bp, 480 aa |
| Immediate neighbours | ywdA, sacP |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
utilization of specific carbon sources
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU38040
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides (according to Swiss-Prot)
- Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P07819
- KEGG entry: [3]
- E.C. number: 3.2.1.26
Additional information
Expression and regulation
- Regulatory mechanism:
- SacT: binding to a RNA switch results in transcription antitermination PubMed
- CcpA: transcription repression
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
M Arnaud, M Débarbouillé, G Rapoport, M H Saier, J Reizer
In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis.
J Biol Chem: 1996, 271(31);18966-72
[PubMed:8702561]
[WorldCat.org]
[DOI]
(P p)
M Debarbouille, M Arnaud, A Fouet, A Klier, G Rapoport
The sacT gene regulating the sacPA operon in Bacillus subtilis shares strong homology with transcriptional antiterminators.
J Bacteriol: 1990, 172(7);3966-73
[PubMed:2163394]
[WorldCat.org]
[DOI]
(P p)
A Fouet, A Klier, G Rapoport
Nucleotide sequence of the sucrase gene of Bacillus subtilis.
Gene: 1986, 45(2);221-5
[PubMed:3100393]
[WorldCat.org]
[DOI]
(P p)
