CarA

• Description: carbamoyl-phosphate transferase-arginine (subunit A)
  
  

Gene name	carA
Synonyms	cpa
Essential	no
Product	carbamoyl-phosphate transferase-arginine (subunit A)
Function	biosynthesis of arginine
Gene expression levels in SubtiExpress: carA
Interactions involving this protein in SubtInteract: CarA
Metabolic function and regulation of this protein in SubtiPathways: Pyrimidines, Ammonium/ glutamate
MW, pI	38 kDa, 6.212
Gene length, protein length	1059 bp, 353 aa
Immediate neighbours	argD, carB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

AhrC regulon

The gene

Basic information

• Locus tag: BSU11230

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 ATP + L-glutamine + HCO₃^- + H₂O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate (according to Swiss-Prot)

• Protein family: glutamine amidotransferase type-1 domain (according to Swiss-Prot)

• Paralogous protein(s): PyrAA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: CarA-CarB

• Localization:

Database entries

• Structure: 1JDB (from Escherichia coli, 47% identity, 64% similarity) PubMed

• UniProt: P36838

• KEGG entry: [3]

• E.C. number: 6.3.5.5

Expression and regulation

• Operon: argC-argJ-argB-argD-carA-carB-argF PubMed

• Expression browser: carA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • repressed by arginine (AhrC) PubMed

• Regulatory mechanism:
  • AhrC: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

A Mountain, N H Mann, R N Munton, S Baumberg
Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis.
Mol Gen Genet: 1984, 197(1);82-9
[PubMed:6096675] [WorldCat.org] [DOI] (P p)