Difference between revisions of "PGP574"

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based on pET3C
 
based on pET3C
  
Schirmer et al. (1997) J. Bacteriol. '''179:''' 1329-1336. [http://www.ncbi.nlm.nih.gov/sites/entrez/9023219 PubMed]
+
Schilling, O., Herzberg, C., Hertrich, T., Vörsmann, H., Jessen, D., Hübner, S., Titgemeyer, F. & Stülke, J. (2006) Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples. Nucl. Acids Res. 34: 6102-6115. [http://www.ncbi.nlm.nih.gov/sites/entrez/17074746 PubMed]

Revision as of 10:32, 15 January 2009

pGP574

vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the C-terminus of the expressed protein

host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Stülke lab

in E. coli: ampicillin resistance

based on pET3C

Schilling, O., Herzberg, C., Hertrich, T., Vörsmann, H., Jessen, D., Hübner, S., Titgemeyer, F. & Stülke, J. (2006) Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples. Nucl. Acids Res. 34: 6102-6115. PubMed