Difference between revisions of "CdaR"
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Revision as of 12:25, 16 May 2013
- Description: effector protein controlling CdaA diadenylate cyclase activity
| Gene name | cdaR |
| Synonyms | ybbR |
| Essential | no |
| Product | effector protein controlling CdaA diadenylate cyclase activity |
| Function | regulation of c-di-AMP synthesis |
| Gene expression levels in SubtiExpress: cdaR | |
| Interactions involving this protein in SubtInteract: CdaR | |
| Metabolic function and regulation of this protein in SubtiPathways: Murein recycling | |
| MW, pI | 52 kDa, 4.719 |
| Gene length, protein length | 1449 bp, 483 aa |
| Immediate neighbours | cdaA, glmM |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
metabolism of signalling nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU01760
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O34659
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
Biological materials
- Mutant:
- GP999 (cdaR::cat), available in Jörg Stülke's lab
- GP985 (cdaA-cdaR::cat), available in Jörg Stülke's lab
- Expression vector:
- IPTG inducible expression of Strep-cdaR in E. coli: pGP2565 (in pGP172), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmids: pGP1989 and pGP1992.
- Strep-tag construct:
- GP1330 (aphA3, based on pGP1460), available in Jörg Stülke's lab
- GP1380 lacA::cdaR-Strep aphA3 glmM-3xFLAG ermC (based on pGP1460 and pGP1087), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
