HolA

• Description: DNA polymerase III, delta subunit, part of the clamp-loader complex and the replisome
  
  

Gene name	holA
Synonyms	yqeN
Essential	yes PubMed
Product	DNA polymerase III, delta subunit
Function	DNA replication
Gene expression levels in SubtiExpress: holA
Interactions involving this protein in SubtInteract: HolA
MW, pI	40 kDa, 6.231
Gene length, protein length	1041 bp, 347 aa
Immediate neighbours	rpsT, yqzM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU25560

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for bacteriophage SPP1 replication PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • domain I: aa 1 - 143, RecA-like fold PubMed
  • domain II: aa 144 - 214, four alpha helices PubMed
  • domain III: aa 215 - 339, seven alpha helices PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:#
  • DnaX(3)-HolA-HolB, the clamp-loader complex PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • DnaE-HolA PubMed

• Localization: Nucleoid (Mid-cell) PubMed

Database entries

• Structure: 3ZH9 PubMed

• UniProt: P54459

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: holA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: