ProS

• Description: prolyl-tRNA synthetase
  
  

Gene name	proS
Synonyms
Essential	yes PubMed
Product	prolyl-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: proS
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	63 kDa, 5.012
Gene length, protein length	1692 bp, 564 aa
Immediate neighbours	rasP, polC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

The gene

Basic information

• Locus tag: BSU16570

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro) (according to Swiss-Prot)

• Protein family: ProS type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2I4L (from Rhodopseudomonas palustris, 51% identity, 71% similarity) PubMed

• UniProt: O31755

• KEGG entry: [2]

• E.C. number: 6.1.1.15

Expression and regulation

• Operon:

• Expression browser: proS PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Thibaut Crepin, Anna Yaremchuk, Mikhail Tukalo, Stephen Cusack
Structures of two bacterial prolyl-tRNA synthetases with and without a cis-editing domain.
Structure: 2006, 14(10);1511-25
[PubMed:17027500] [WorldCat.org] [DOI] (P p)