YabG

• Description: protease
  
  

Gene name	yabG
Synonyms
Essential	no
Product	protease
Function	modification of spore coat proteins
Gene expression levels in SubtiExpress: yabG
MW, pI	33 kDa, 9.143
Gene length, protein length	870 bp, 290 aa
Immediate neighbours	ksgA, veg
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU00430

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: temperature-dependent modification of the coat proteins such as GerQ (together with Tgl) PubMed

• Protein family: peptidase U57 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: forespore outer membrane (according to Swiss-Prot), spore coat PubMed

Database entries

• Structure:

• UniProt: P37548

• KEGG entry: [3]

• E.C. number:

Additional information

colocalizes in the spore coat with YeeK PubMed

Expression and regulation

• Operon: yabG PubMed

• Expression browser: yabG PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S128 ( yabG::erm), PubMed, available at BGSC
  • 1S128 ( yabG::erm), PubMed, available at BGSC
  • 1A814 ( yabG::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Ritsuko Kuwana
[Protein modification system in dormant cells].
Yakugaku Zasshi: 2009, 129(10);1221-5
[PubMed:19797877] [WorldCat.org] [DOI] (P p)

Original Publications

Hiromu Takamatsu, Daisuke Imamura, Ritsuko Kuwana, Kazuhito Watabe
Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy.
J Bacteriol: 2009, 191(4);1220-9
[PubMed:19060142] [WorldCat.org] [DOI] (I p)

Ritsuko Kuwana, Naoyuki Okuda, Hiromu Takamatsu, Kazuhito Watabe
Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores.
J Biochem: 2006, 139(5);887-901
[PubMed:16751597] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

H Takamatsu, T Kodama, A Imamura, K Asai, K Kobayashi, T Nakayama, N Ogasawara, K Watabe
The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition.
J Bacteriol: 2000, 182(7);1883-8
[PubMed:10714992] [WorldCat.org] [DOI] (P p)