Difference between revisions of "RecA"
| Line 85: | Line 85: | ||
* '''Modification:''' | * '''Modification:''' | ||
** phosphorylated on Arg-58 {{PubMed|22517742}} | ** phosphorylated on Arg-58 {{PubMed|22517742}} | ||
| − | ** phosphorylated on Ser-2 {{PubMed|20509597}} | + | ** phosphorylated on Ser-2 {{PubMed|20509597}} by [[YabT]] {{PubMed|23634894}} |
* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
| Line 97: | Line 97: | ||
** [[RecA]]-[[ComFA]] {{PubMed|17630974}} | ** [[RecA]]-[[ComFA]] {{PubMed|17630974}} | ||
** [[SsbB]]-[[RecA]] {{PubMed|17630974}} | ** [[SsbB]]-[[RecA]] {{PubMed|17630974}} | ||
| + | ** [[YabT]]-[[RecA]] {{PubMed|23634894}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| Line 102: | Line 103: | ||
** Nucleoid (Mid-cell) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] | ** Nucleoid (Mid-cell) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] | ||
** localizes to one cell pole {{PubMed|21278288}} | ** localizes to one cell pole {{PubMed|21278288}} | ||
| + | ** forms a transient, mobile focus associated with the chromosome during spore development {{PubMed|23634894}} | ||
=== Database entries === | === Database entries === | ||
| Line 135: | Line 137: | ||
=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' IRN444 (cat), available in [[Jörg Stülke]]'s lab | + | * '''Mutant:''' |
| + | ** IRN444 (cat), available in [[Jörg Stülke]]'s lab | ||
** 1A746 ( ''recA''::''erm''), {{PubMed|1391055}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A746&Search=1A746 BGSC] | ** 1A746 ( ''recA''::''erm''), {{PubMed|1391055}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A746&Search=1A746 BGSC] | ||
** 1A786 ( ''recA''::''kan''), {{PubMed|11208805}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A786&Search=1A786 BGSC] | ** 1A786 ( ''recA''::''kan''), {{PubMed|11208805}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A786&Search=1A786 BGSC] | ||
| − | * '''Expression vector:''' for expression, purification in ''E. coli'' with N-terminal His-tag, pRSETA available in Gerth lab | + | * '''Expression vector:''' for expression, purification in ''E. coli'' with N-terminal His-tag, pRSETA available in [[Ulf Gerth]]'s lab |
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
| Line 160: | Line 163: | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>11814663,16061691,19060143,17803906,16024744,17630974,8226626,11555642, 16479537 19730681 7690748 17229847,16267290 20509597 20723756 17449621 21278288 22373918 22517742 23284295 23536821 21859751</pubmed> | + | <pubmed>11814663,16061691,19060143,17803906,16024744,17630974,8226626,11555642, 16479537 19730681 7690748 17229847,16267290 20509597 20723756 17449621 21278288 22373918 22517742 23284295 23536821 21859751 23634894</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 18:41, 6 May 2013
- Description: multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage)
| Gene name | recA |
| Synonyms | recE |
| Essential | no |
| Product | multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage) |
| Function | DNA repair/ recombination |
| Gene expression levels in SubtiExpress: recA | |
| Interactions involving this protein in SubtInteract: RecA | |
| MW, pI | 37 kDa, 4.883 |
| Gene length, protein length | 1041 bp, 347 aa |
| Immediate neighbours | cinA, pbpX |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA repair/ recombination, genetic competence, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16940
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
RecA filaments are dismantled from DNA by PcrA PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: recA family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 1U94 (RecA from E. coli, 62% identity, 86% similarity)
- UniProt: P16971
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: recA PubMed
- Regulation:
- Additional information:
Biological materials
- Mutant:
- IRN444 (cat), available in Jörg Stülke's lab
- 1A746 ( recA::erm), PubMed, available at BGSC
- 1A786 ( recA::kan), PubMed, available at BGSC
- Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Peter Graumann, Freiburg University, Germany homepage
Your additional remarks
References
Reviews
Juan C Alonso, Paula P Cardenas, Humberto Sanchez, James Hejna, Yuki Suzuki, Kunio Takeyasu
Early steps of double-strand break repair in Bacillus subtilis.
DNA Repair (Amst): 2013, 12(3);162-76
[PubMed:23380520]
[WorldCat.org]
[DOI]
(I p)
Dawit Kidane, Silvia Ayora, Joann B Sweasy, Peter L Graumann, Juan C Alonso
The cell pole: the site of cross talk between the DNA uptake and genetic recombination machinery.
Crit Rev Biochem Mol Biol: 2012, 47(6);531-55
[PubMed:23046409]
[WorldCat.org]
[DOI]
(I p)
Silvia Ayora, Begoña Carrasco, Paula P Cárdenas, Carolina E César, Cristina Cañas, Tribhuwan Yadav, Chiara Marchisone, Juan C Alonso
Double-strand break repair in bacteria: a view from Bacillus subtilis.
FEMS Microbiol Rev: 2011, 35(6);1055-81
[PubMed:21517913]
[WorldCat.org]
[DOI]
(I p)
Justin Courcelle, Philip C Hanawalt
RecA-dependent recovery of arrested DNA replication forks.
Annu Rev Genet: 2003, 37;611-46
[PubMed:14616075]
[WorldCat.org]
[DOI]
(P p)
Michael M Cox
The bacterial RecA protein as a motor protein.
Annu Rev Microbiol: 2003, 57;551-77
[PubMed:14527291]
[WorldCat.org]
[DOI]
(P p)
Shelley L Lusetti, Michael M Cox
The bacterial RecA protein and the recombinational DNA repair of stalled replication forks.
Annu Rev Biochem: 2002, 71;71-100
[PubMed:12045091]
[WorldCat.org]
[DOI]
(P p)
M M Cox
Recombinational DNA repair in bacteria and the RecA protein.
Prog Nucleic Acid Res Mol Biol: 1999, 63;311-66
[PubMed:10506835]
[WorldCat.org]
[DOI]
(P p)
Original publications
