DnaX

• Description: DNA polymerase III (gamma and tau subunits) part of the clamp-loader complex and the replisome
  
  

Gene name	dnaX
Synonyms	dnaH, dna-8132
Essential	yes PubMed
Product	DNA polymerase III (gamma and tau subunits)
Function	DNA replication
Gene expression levels in SubtiExpress: dnaX
Interactions involving this protein in SubtInteract: DnaX
MW, pI	62 kDa, 5.488
Gene length, protein length	1689 bp, 563 aa
Immediate neighbours	scr, yaaK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU00190

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • required for bacteriophage SPP1 replication PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DnaX-DnaB PubMed
  • DnaX-DnaC PubMed
  • DnaX(3)-HolA-HolB, the clamp-loader complex PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed

• Localization:
  • forms foci PubMed
  • Nucleoid (Mid-cell) PubMed

Database entries

• Structure:

• UniProt: P09122

• KEGG entry: [2]

• E.C. number: 2.7.7.7

Expression and regulation

• Operon:

• Expression browser: dnaX PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: