Difference between revisions of "YngF"

From SubtiWiki
Jump to: navigation, search
Line 14: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || mother cell metabolism, leucine utilization
 
|style="background:#ABCDEF;" align="center"|'''Function''' || mother cell metabolism, leucine utilization
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU18220 yngF]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18220 yngF]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 7.671   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 7.671   
Line 22: Line 22:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yngE]]'', ''[[yngG]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yngE]]'', ''[[yngG]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13705&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU18220 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU18220 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU18220 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yngF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yngF_context.gif]]

Revision as of 12:57, 13 May 2013

  • Description: methylglutaconyl-CoA hydratase

Gene name yngF
Synonyms
Essential no
Product methylglutaconyl-CoA hydratase
Function mother cell metabolism, leucine utilization
Gene expression levels in SubtiExpress: yngF
MW, pI 27 kDa, 7.671
Gene length, protein length 780 bp, 260 aa
Immediate neighbours yngE, yngG
Sequences Protein DNA Advanced_DNA
Genetic context
YngF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YngF expression.png















Categories containing this gene/protein

utilization of amino acids, sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

  • Locus tag: BSU18220

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 3-methylglutaconyl-CoA --->(S)-3-hydroxy-3-methylglutaryl-CoA PubMed
  • Protein family: enoyl-CoA hydratase/isomerase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3KQF (from B. anthracis)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed during sporulation in the mother cell (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Tzu-Lin Hsiao, Olga Revelles, Lifeng Chen, Uwe Sauer, Dennis Vitkup
Automatic policing of biochemical annotations using genomic correlations.
Nat Chem Biol: 2010, 6(1);34-40
[PubMed:19935659] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)