Difference between revisions of "CsrA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || control of ''[[hag]]'' translation  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of ''[[hag]]'' translation  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU35370 csrA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35370 csrA]
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CsrA CsrA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CsrA CsrA]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hag]]'', ''[[fliW]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hag]]'', ''[[fliW]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15554&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35370 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35370 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35370 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:csrA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:csrA_context.gif]]

Revision as of 13:59, 13 May 2013

  • Description: motility regulator, binds to the hag mRNA to inhibit its translation

Gene name csrA
Synonyms yviG
Essential no
Product motility regulator
Function control of hag translation
Gene expression levels in SubtiExpress: csrA
Interactions involving this protein in SubtInteract: CsrA
MW, pI 8 kDa, 6.09
Gene length, protein length 222 bp, 74 aa
Immediate neighbours hag, fliW
Sequences Protein DNA Advanced_DNA
Genetic context
CsrA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CsrA expression.png



















Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

The CsrA regulon: hag

The gene

Basic information

  • Locus tag: BSU35370

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: csrA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • CsrA is sequestered by interaction with FliW (the two proteins form a conserved module in many bacteria) PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP469 (spc), available in Stülke lab
  • Expression vector:
    • pGP381 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP383, available in Stülke lab
  • lacZ fusion: pGP461 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Additional Reviews: PubMed

Original Publications