CdaR

• Description: unknown
  
  

Gene name	ybbR
Synonyms
Essential	no
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: ybbR
Metabolic function and regulation of this protein in SubtiPathways: Murein recycling
MW, pI	52 kDa, 4.719
Gene length, protein length	1449 bp, 483 aa
Immediate neighbours	ybbP, glmM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteins of unknown function

The gene

Basic information

• Locus tag: BSU01760

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34659

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ybbP-ybbR-glmM-glmS

• Expression browser: ybbR PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information: the ybbP-ybbR part of the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:
  • GP999 (ybbR::cat), available in Jörg Stülke's lab
  • GP985 (ybbP-ybbR::cat), available in Jörg Stülke's lab

• Expression vector:
  • IPTG inducible expression of Strep-ybbR in E. coli: pGP2565 (in pGP172), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmids: pGP1989 and pGP1992.

• Strep-tag construct:
  • GP1330 (aphA3, based on pGP1460), available in Jörg Stülke's lab

• Antibody:

References

Additional publications: PubMed

Jun Yin, Paul D Straight, Shaun M McLoughlin, Zhe Zhou, Alison J Lin, David E Golan, Neil L Kelleher, Roberto Kolter, Christopher T Walsh
Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase.
Proc Natl Acad Sci U S A: 2005, 102(44);15815-20
[PubMed:16236721] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)