Difference between revisions of "GapB"
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|style="background:#ABCDEF;" align="center"|'''Function''' || anabolic enzyme in gluconeogenesis | |style="background:#ABCDEF;" align="center"|'''Function''' || anabolic enzyme in gluconeogenesis | ||
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− | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29020 gapB] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Cys, Met & Sulfate assimilation], [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Cys, Met & Sulfate assimilation], [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[speD]]'', ''[[ytcD]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[speD]]'', ''[[ytcD]]'' | ||
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− | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29020 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29020 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29020 Advanced_DNA] |
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|colspan="2" | '''Genetic context''' <br/> [[Image:gapB_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:gapB_context.gif]] |
Revision as of 13:33, 13 May 2013
- Description: glyceraldehyde-3-phosphate dehydrogenase, NADP-dependent, gluconeogenic enzyme, forms a transhydrogenation cycle with GapA for balancing of NADPH
Gene name | gapB |
Synonyms | ppc |
Essential | no |
Product | glyceraldehyde-3-phosphate dehydrogenase 2 |
Function | anabolic enzyme in gluconeogenesis |
Gene expression levels in SubtiExpress: gapB | |
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation, Central C-metabolism | |
MW, pI | 37,3 kDa, 6.47 |
Gene length, protein length | 1020 bp, 340 amino acids |
Immediate neighbours | speD, ytcD |
Sequences | Protein DNA Advanced_DNA |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29020
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H (according to Swiss-Prot)
- This reaction is part of the gluconeogenesis
- Protein family: glyceraldehyde-3-phosphate dehydrogenase family (according to Swiss-Prot)
- Paralogous protein(s): GapA
Extended information on the protein
- Kinetic information: Michaelis-Menten PubMed
- Domains:
- Nucleotid bindinge domain (12-13)
- 2x Glyceraldehyde 3-phosphate binding domain (151-153) & (210-211)
- Modification:
- Cofactor(s): NADP (preferentially) and NAD PubMed
- Effectors of protein activity:
- Localization: Cytoplasm (Homogeneous) PubMed
Database entries
- Structure: 3PRL (from B. halodurans)
- UniProt: O34425
- KEGG entry: [3]
- E.C. number: 1.2.1.59
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Your additional remarks
References
Additional publications: PubMed