HlpB

• Description: HNH nuclease-like protein, rescues AddA-AddB-mediated recombination intermediates
  
  

Gene name	hlpB
Synonyms	yisB
Essential	no
Product	HNH nuclease-like protein
Function	rescuing of AddA-AddB-mediated recombination intermediates
Gene expression levels in SubtiExpress: yisB
MW, pI	11 kDa, 9.941
Gene length, protein length	300 bp, 100 aa
Immediate neighbours	sbcC, gerPF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU10660

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O06715

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sbcD-sbcC-yisB (according to DBTBS)

• Expression browser: yisB PubMed

• Sigma factor:

• Regulation:
  • positive regulation by ComK PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Miriam Pediaditakis, Miriam Kaufenstein, Peter L Graumann
Bacillus subtilis hlpB encodes a conserved stand-alone HNH nuclease-like protein that is essential for viability unless the hlpB deletion is accompanied by the deletion of genes encoding the AddAB DNA repair complex.
J Bacteriol: 2012, 194(22);6184-94
[PubMed:22984257] [WorldCat.org] [DOI] (I p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)