YabG

• Description: protease
  
  

Gene name	yabG
Synonyms
Essential	no
Product	protease
Function	modification of spore coat proteins
Gene expression levels in SubtiExpress: yabG
MW, pI	33 kDa, 9.143
Gene length, protein length	870 bp, 290 aa
Immediate neighbours	ksgA, veg
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU00430

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: temperature-dependent modification of the coat proteins such as GerQ (together with Tgl) PubMed

• Protein family: peptidase U57 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: forespore outer membrane (according to Swiss-Prot), spore coat PubMed

Database entries

• Structure:

• UniProt: P37548

• KEGG entry: [3]

• E.C. number:

Additional information

colocalizes in the spore coat with YeeK PubMed

Expression and regulation

• Operon: yabG PubMed

• Expression browser: yabG PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S128 ( yabG::erm), PubMed, available at BGSC
  • 1S128 ( yabG::erm), PubMed, available at BGSC
  • 1A814 ( yabG::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Ritsuko Kuwana
[Protein modification system in dormant cells].
Yakugaku Zasshi: 2009, 129(10);1221-5
[PubMed:19797877] [WorldCat.org] [DOI] (P p)

Original Publications

Hiromu Takamatsu, Daisuke Imamura, Ritsuko Kuwana, Kazuhito Watabe
Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy.
J Bacteriol: 2009, 191(4);1220-9
[PubMed:19060142] [WorldCat.org] [DOI] (I p)

Ritsuko Kuwana, Naoyuki Okuda, Hiromu Takamatsu, Kazuhito Watabe
Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores.
J Biochem: 2006, 139(5);887-901
[PubMed:16751597] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

H Takamatsu, T Kodama, A Imamura, K Asai, K Kobayashi, T Nakayama, N Ogasawara, K Watabe
The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition.
J Bacteriol: 2000, 182(7);1883-8
[PubMed:10714992] [WorldCat.org] [DOI] (P p)