Difference between revisions of "YabA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || positioning of [[DnaA]]
 
|style="background:#ABCDEF;" align="center"|'''Function''' || positioning of [[DnaA]]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00330 yabA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00330 yabA]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/YabA YabA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/YabA YabA]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yaaT]]'', ''[[yabB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yaaT]]'', ''[[yabB]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11809&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00330 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00330 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00330 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yabA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yabA_context.gif]]

Revision as of 11:54, 13 May 2013

  • Description: tethers DnaA to the polymerase-clamp protein DnaN

Gene name yabA
Synonyms
Essential no
Product negative regulator of replication initiation
Function positioning of DnaA
Gene expression levels in SubtiExpress: yabA
Interactions involving this protein in SubtInteract: YabA
MW, pI 13 kDa, 6.065
Gene length, protein length 357 bp, 119 aa
Immediate neighbours yaaT, yabB
Sequences Protein DNA Advanced_DNA
Genetic context
YabA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YabA expression.png
























Categories containing this gene/protein

DNA replication

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00330

Phenotypes of a mutant

strongly reduced growth rate in minimal medium PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • negative control of replication initiation PubMed
    • associates with the oriC region in a DnaA-dependent manner, this limits the amount of DnaA that can bind the oriC region PubMed
    • YabA inhibits co-operative DNA binding by DnaA PubMed
  • Protein family: yabA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: leucine zipper
  • Modification:
  • Cofactor(s): Zn cluster
  • Effectors of protein activity:
    • DnaN overexpression or release from the replisome decreases association of YabA with oriC, increases association of DnaA with oriC PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Philippe Noirot, Jouy-en-Josas, France Homepage
Alan Grossman, Cambridge, MA, USA Homepage

Your additional remarks

References

Reviews

Tsutomu Katayama, Shogo Ozaki, Kenji Keyamura, Kazuyuki Fujimitsu
Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC.
Nat Rev Microbiol: 2010, 8(3);163-70
[PubMed:20157337] [WorldCat.org] [DOI] (I p)

Original publications