Difference between revisions of "CapE"
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|''capE'' | |''capE'' | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywtC, pgsE'' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywtC, pgsE, edmS'' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
| − | |||
| − | |||
| − | |||
| − | |||
<br/><br/> | <br/><br/> | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[capsule biosynthesis and degradation]]}} | + | {{SubtiWiki category|[[capsule biosynthesis and degradation]]}}, |
| + | {{SubtiWiki category|[[membrane proteins]]}} | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| Line 70: | Line 67: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' stimulates poly-gamma-glutamate production in the presence of zinc {{PubMed|20812257}} | + | * '''Catalyzed reaction/ biological activity:''' |
| + | ** stimulates poly-gamma-glutamate production in the presence of zinc {{PubMed|20812257}} | ||
| + | ** also involved in extra-chromosomal DNA maintenance {{PubMed|23583563,21357437}} | ||
* '''Protein family:''' | * '''Protein family:''' | ||
| Line 80: | Line 79: | ||
* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''Domains:''' N-terminal membrane-anchoring domain and a C-terminal assembly accelerator-like structure {{PubMed|23583563}} |
* '''Modification:''' | * '''Modification:''' | ||
| Line 91: | Line 90: | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| + | ** cell membrane {{PubMed|23583563}} | ||
=== Database entries === | === Database entries === | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pgsE_3697639_3697806_-1 capE] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pgsE_3697639_3697806_-1 capE] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19420703 PubMed] | + | * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19420703 PubMed] |
* '''Regulation:''' | * '''Regulation:''' | ||
| Line 137: | Line 137: | ||
=References= | =References= | ||
| − | + | <pubmed>19420703, 16233197 16267300 20564357 20564574 21357437 23583563 20812257,21965392</pubmed> | |
| − | <pubmed>19420703, 16233197 16267300 20564357 20564574 21357437</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:55, 19 April 2013
- Description: stimulates poly-gamma-glutamate production in the presence of zinc, also involved in extra-chromosomal DNA maintenance
| Gene name | capE |
| Synonyms | ywtC, pgsE, edmS |
| Essential | no |
| Product | unknown |
| Function | capsule synthesis |
| Gene expression levels in SubtiExpress: capE | |
| MW, pI | 6 kDa, 6.487 |
| Gene length, protein length | 165 bp, 55 aa |
| Immediate neighbours | pgdS, capA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
capsule biosynthesis and degradation, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU35870
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains: N-terminal membrane-anchoring domain and a C-terminal assembly accelerator-like structure PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane PubMed
Database entries
- Structure:
- UniProt: P96739
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information: The operon is not expressed in the lab strain 168 due to lack of expression of DegQ resulting in inactivity of DegS and DegU PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Makoto Ashiuchi, Daisuke Yamashiro, Kento Yamamoto
Bacillus subtilis EdmS (formerly PgsE) participates in the maintenance of episomes.
Plasmid: 2013, 70(2);209-15
[PubMed:23583563]
[WorldCat.org]
[DOI]
(I p)
Thi-Huyen Do, Yuki Suzuki, Naoki Abe, Jun Kaneko, Yoshifumi Itoh, Keitarou Kimura
Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis.
Appl Environ Microbiol: 2011, 77(23);8249-58
[PubMed:21965392]
[WorldCat.org]
[DOI]
(I p)
Daisuke Yamashiro, Yutaka Minouchi, Makoto Ashiuchi
Moonlighting role of a poly-gamma-glutamate synthetase component from Bacillus subtilis: insight into novel extrachromosomal DNA maintenance.
Appl Environ Microbiol: 2011, 77(8);2796-8
[PubMed:21357437]
[WorldCat.org]
[DOI]
(I p)
Daisuke Yamashiro, Megumi Yoshioka, Makoto Ashiuchi
Bacillus subtilis pgsE (Formerly ywtC) stimulates poly-γ-glutamate production in the presence of zinc.
Biotechnol Bioeng: 2011, 108(1);226-30
[PubMed:20812257]
[WorldCat.org]
[DOI]
(I p)
Tohru Kamei, Daisuke Yamashiro, Terumi Horiuchii, Yutaka Minouchi, Makoto Ashiuchi
Identification and biochemical characterization of membranous short-chain polyglutamate from Bacillus subtilis.
Chem Biodivers: 2010, 7(6);1563-72
[PubMed:20564574]
[WorldCat.org]
[DOI]
(I p)
Chuan-Mei Yeh, Jyh-Perng Wang, Shih-Ching Lo, Wen-Chia Chan, Ming-Yi Lin
Chromosomal integration of a synthetic expression control sequence achieves poly-gamma-glutamate production in a Bacillus subtilis strain.
Biotechnol Prog: 2010, 26(4);1001-7
[PubMed:20564357]
[WorldCat.org]
[DOI]
(I p)
Keitarou Kimura, Lam-Son Phan Tran, Thi-Huyen Do, Yoshifumi Itoh
Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis (natto).
Biosci Biotechnol Biochem: 2009, 73(5);1149-55
[PubMed:19420703]
[WorldCat.org]
[DOI]
(I p)
Thomas Candela, Michèle Mock, Agnès Fouet
CapE, a 47-amino-acid peptide, is necessary for Bacillus anthracis polyglutamate capsule synthesis.
J Bacteriol: 2005, 187(22);7765-72
[PubMed:16267300]
[WorldCat.org]
[DOI]
(P p)
Yuji Urushibata, Shinji Tokuyama, Yasutaka Tahara
Difference in transcription levels of cap genes for gamma-polyglutamic acid production between Bacillus subtilis IFO 16449 and Marburg 168.
J Biosci Bioeng: 2002, 93(2);252-4
[PubMed:16233197]
[WorldCat.org]
[DOI]
(P p)
