PheS

• Description: phenylalanyl-tRNA synthetase (alpha subunit), universally conserved protein
  
  

Gene name	pheS
Synonyms
Essential	yes PubMed
Product	phenylalanyl-tRNA synthetase (alpha subunit)
Function	translation
Gene expression levels in SubtiExpress: pheS
Interactions involving this protein in SubtInteract: PheS
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	38 kDa, 5.083
Gene length, protein length	1032 bp, 344 aa
Immediate neighbours	pheT, ysgA
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, universally conserved proteins

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU28640

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)

• Protein family: Phe-tRNA synthetase alpha chain type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: PheS-PheT

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1PYS (from Thermus thermophilus hb8 mutant, 37% identity, 56% similarity) PubMed

• UniProt: P17921

• KEGG entry: [3]

• E.C. number: 6.1.1.20

Additional information

universally conserved protein

Expression and regulation

• Operon: pheS-pheT PubMed

• Expression browser: pheS PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by phenylalanine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

A A Brakhage, M Wozny, H Putzer
Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.
Biochimie: 1990, 72(10);725-34
[PubMed:2127701] [WorldCat.org] [DOI] (P p)