SdhB

• Description: succinate dehydrogenase
  
  

Gene name	sdhB
Synonyms
Essential	no
Product	succinate dehydrogenase (iron-sulfur protein)
Function	TCA cycle
Gene expression levels in SubtiExpress: sdhB
Interactions involving this protein in SubtInteract: SdhB
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	28 kDa, 7.989
Gene length, protein length	759 bp, 253 aa
Immediate neighbours	ysmA, sdhA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, membrane proteins

This gene is a member of the following regulons

FsrA regulon

The gene

Basic information

• Locus tag: BSU28430

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Succinate + acceptor = fumarate + reduced acceptor (according to Swiss-Prot)

• Protein family: succinate dehydrogenase/fumarate reductase iron-sulfur protein family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Fe

• Effectors of protein activity:
  • Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide PubMed
  • Activated by Cytochrome b558 PubMed

• Interactions:
  • SdhA-SdhB, SdhB-SdhC (as part of the SdhC-SdhB-SdhA complex)

• Localization: attached to the membrane PubMed

Database entries

• Structure: 1NEK (E. coli)

• UniProt: P08066

• KEGG entry: [3]

• E.C. number:EC 1.3.99.1

Additional information

• This enzyme is a trimer membrane-bound PubMed PubMed
  • One subunit is bound to citochrome b558, and this subunit is the one bound to the cytosolic side of the membrane PubMed PubMed
  • Another subunit is the flavoprotein one, required for FAD usage PubMed PubMed
  • The other subunit has an iron-sulphur domain necessary for the catalytic activity PubMed PubMed
• extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at Proteopedia

Expression and regulation

• Operon: sdhC-sdhA-sdhB PubMed

• Expression browser: sdhB PubMed

• Sigma factor: SigA PubMed

• Regulation: constitutive
  • part of the iron sparing response (FsrA) PubMed

• Regulatory mechanism:
  • FsrA: translation inhibition PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

• FLAG-tag construct: GP1426 (spc, based on pGP1331), available in the Stülke lab