CymR

• Description: pleiotropic regulator of sulfur metabolism
  
  

Gene name	cymR
Synonyms	yrzC
Essential	no
Product	transcription repressor
Function	regulation of sulfur metabolism
Gene expression levels in SubtiExpress: cymR
Interactions involving this protein in SubtInteract: CymR
Metabolic function and regulation of this protein in SubtiPathways: Riboflavin / FAD, Cys, Met & Sulfate assimilation
MW, pI	11 kDa, 9.879
Gene length, protein length	333 bp, 111 aa
Immediate neighbours	yrvO, yrvN
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sulfur metabolism, transcription factors and their control, regulators of core metabolism

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU27520

Phenotypes of a mutant

• impaired growth in the presence of cystine and increased sensitivity to hydrogen peroxide-, disulfide-, paraquat- and tellurite-induced stresses PubMed
• increased intracellular cysteine pool and hydrogen sulfide formation PubMed
• depletion of branched-chain amino acids PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: Rrf2 family of transcription regulators

• Paralogous protein(s):

Genes repressed by CymR

• ytmI-tcyJ-tcyK-tcyL-tcyM-tcyN-ytmO-ytnI-ytnJ-ribR-ytnL-ytnM PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): CysK acts as corepressor PubMed

• Effectors of protein activity:

• Interactions:
  • CymR-CysK, the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2Y75 PubMed

• UniProt: O34527

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cymR-yrvO-trmU PubMed

• Expression browser: cymR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

References

The CymR regulon

Sergine Even, Pierre Burguière, Sandrine Auger, Olga Soutourina, Antoine Danchin, Isabelle Martin-Verstraete
Global control of cysteine metabolism by CymR in Bacillus subtilis.
J Bacteriol: 2006, 188(6);2184-97
[PubMed:16513748] [WorldCat.org] [DOI] (P p)

Other original publications

Additional publications: PubMed

Marie-Françoise Hullo, Isabelle Martin-Verstraete, Olga Soutourina
Complex phenotypes of a mutant inactivated for CymR, the global regulator of cysteine metabolism in Bacillus subtilis.
FEMS Microbiol Lett: 2010, 309(2);201-7
[PubMed:20608979] [WorldCat.org] [DOI] (I p)

Catherine Tanous, Olga Soutourina, Bertrand Raynal, Marie-Françoise Hullo, Peggy Mervelet, Anne-Marie Gilles, Philippe Noirot, Antoine Danchin, Patrick England, Isabelle Martin-Verstraete
The CymR regulator in complex with the enzyme CysK controls cysteine metabolism in Bacillus subtilis.
J Biol Chem: 2008, 283(51);35551-60
[PubMed:18974048] [WorldCat.org] [DOI] (P p)

Marie-Françoise Hullo, Sandrine Auger, Olga Soutourina, Octavian Barzu, Mireille Yvon, Antoine Danchin, Isabelle Martin-Verstraete
Conversion of methionine to cysteine in Bacillus subtilis and its regulation.
J Bacteriol: 2007, 189(1);187-97
[PubMed:17056751] [WorldCat.org] [DOI] (P p)

Soon-Yong Choi, Dindo Reyes, Montira Leelakriangsak, Peter Zuber
The global regulator Spx functions in the control of organosulfur metabolism in Bacillus subtilis.
J Bacteriol: 2006, 188(16);5741-51
[PubMed:16885442] [WorldCat.org] [DOI] (P p)

Pierre Burguière, Juliette Fert, Isabelle Guillouard, Sandrine Auger, Antoine Danchin, Isabelle Martin-Verstraete
Regulation of the Bacillus subtilis ytmI operon, involved in sulfur metabolism.
J Bacteriol: 2005, 187(17);6019-30
[PubMed:16109943] [WorldCat.org] [DOI] (P p)

Irina M Solovieva, Rimma A Kreneva, Lubov Errais Lopes, Daniel A Perumov
The riboflavin kinase encoding gene ribR of Bacillus subtilis is a part of a 10 kb operon, which is negatively regulated by the yrzC gene product.
FEMS Microbiol Lett: 2005, 243(1);51-8
[PubMed:15668000] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)