Difference between revisions of "Fni"

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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 636 bp, 212 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 636 bp, 212 aa  
 
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yphA]]'', ''[[ypfD]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ypzI]]'', ''[[ypfD]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14203&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14203&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''

Revision as of 13:42, 3 December 2012

  • Description: isopentenyl diphosphate isomerase

Gene name fni
Synonyms ypgA
Essential no
Product isopentenyl diphosphate isomerase
Function lipid metabolism
Gene expression levels in SubtiExpress: fni
MW, pI 22 kDa, 6.101
Gene length, protein length 636 bp, 212 aa
Immediate neighbours ypzI, ypfD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YpgA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Fni expression.png
























Categories containing this gene/protein

biosynthesis of lipids, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22870

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Isopentenyl diphosphate = dimethylallyl diphosphate (according to Swiss-Prot)
  • Protein family: IPP isomerase type 2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1P0N (complex with FMN), 1P0K
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)

Motoki Takagi, Kazuhide Kaneda, Tomohiro Shimizu, Yoichi Hayakawa, Haruo Seto, Tomohisa Kuzuyama
Bacillus subtilis ypgA gene is fni, a nonessential gene encoding type 2 isopentenyl diphosphate isomerase.
Biosci Biotechnol Biochem: 2004, 68(1);132-7
[PubMed:14745175] [WorldCat.org] [DOI] (P p)

Stefan Steinbacher, Johannes Kaiser, Stefan Gerhardt, Wolfgang Eisenreich, Robert Huber, Adelbert Bacher, Felix Rohdich
Crystal structure of the type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase from Bacillus subtilis.
J Mol Biol: 2003, 329(5);973-82
[PubMed:12798687] [WorldCat.org] [DOI] (P p)