Difference between revisions of "BshA"

From SubtiWiki
Jump to: navigation, search
Line 14: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of bacillithiol
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of bacillithiol
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU22460 bshA]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22460 bshA]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 6.149   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 6.149   
Line 22: Line 22:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cca]]'', ''[[bshB1]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cca]]'', ''[[bshB1]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14162&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22460 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22460 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22460 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ypjH_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ypjH_context.gif]]

Revision as of 13:08, 13 May 2013

  • Description: L-malic acid glycosyltransferase, involved in bacillithiol synthesis

Gene name bshA
Synonyms jojH, ypjH
Essential no
Product L-malic acid glycosyltransferase
Function biosynthesis of bacillithiol
Gene expression levels in SubtiExpress: bshA
MW, pI 41 kDa, 6.149
Gene length, protein length 1131 bp, 377 aa
Immediate neighbours cca, bshB1
Sequences Protein DNA Advanced_DNA
Genetic context
YpjH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BshA expression.png
























Categories containing this gene/protein

miscellaneous metabolic pathways, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22460

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: UDP-GlcNAc + L-malate = GlcNAc(α1→2)L-malate PubMed; also uses D-malate as a substrate, but with much lower affinity PubMed
  • Protein family: NamA subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: bshA::mls available in John_Helmann lab
    • GP88 (ypjH::pX2(cat)), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional references: PubMed