Difference between revisions of "PycA"
Line 131: | Line 131: | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
+ | ** pGP1289 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab | ||
* '''lacZ fusion:''' | * '''lacZ fusion:''' |
Revision as of 12:02, 1 October 2012
- Description: pyruvate carboxylase
Gene name | pycA |
Synonyms | ylaP |
Essential | no |
Product | pyruvate carboxylase |
Function | replenishment of the oxaloacetate pool |
Gene expression levels in SubtiExpress: pycA | |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Biotin | |
MW, pI | 127 kDa, 5.407 |
Gene length, protein length | 3444 bp, 1148 aa |
Immediate neighbours | ftsW, ctaA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
carbon core metabolism, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU14860
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): biotin
- Effectors of protein activity:
- Localization: membrane associated PubMed
Database entries
- Structure: 3BG5 (S. aureus)
- UniProt: Q9KWU4
- KEGG entry: [3]
- E.C. number: 6.4.1.1
Additional information
PycA binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by SPINE.
PycA is subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Regulation:
- subject to positive stringent control upon lysine starvation PubMed
- Regulatory mechanism:
- stringent response: due to presence of adenines at +1 and +2 positions of the transcript PubMed
- Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications