Difference between revisions of "IspA"
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= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| − | + | {{SubtiWiki regulon|[[CodY regulon]]}} | |
=The gene= | =The gene= | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ispA_1386024_1386983_-1 ispA] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ispA_1386024_1386983_-1 ispA] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigA]] {{PubMed|3087947}} | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|3087947}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) {{PubMed|23569278}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[CodY]]: transcription repression {{PubMed|23569278}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
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=References= | =References= | ||
| − | <pubmed> 3087947 </pubmed> | + | <pubmed> 3087947 23569278 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 08:33, 19 April 2013
- Description: intracellular serine protease
| Gene name | ispA |
| Synonyms | |
| Essential | no |
| Product | intracellular serine protease |
| Function | protein degradation |
| Gene expression levels in SubtiExpress: ispA | |
| MW, pI | 33 kDa, 4.522 |
| Gene length, protein length | 957 bp, 319 aa |
| Immediate neighbours | metE, rsbRB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
utilization of nitrogen sources other than amino acids, proteolysis
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13190
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: peptidase S8 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P11018
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: ispA (according to DBTBS)
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Boris R Belitsky, Abraham L Sonenshein
Genome-wide identification of Bacillus subtilis CodY-binding sites at single-nucleotide resolution.
Proc Natl Acad Sci U S A: 2013, 110(17);7026-31
[PubMed:23569278]
[WorldCat.org]
[DOI]
(I p)
Y Koide, A Nakamura, T Uozumi, T Beppu
Cloning and sequencing of the major intracellular serine protease gene of Bacillus subtilis.
J Bacteriol: 1986, 167(1);110-6
[PubMed:3087947]
[WorldCat.org]
[DOI]
(P p)
