Difference between revisions of "Ctc"

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* '''Description:''' general stress protein, similar to [[ribosomal protein]] L25 <br/><br/>
  
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || unknown  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown  
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00520 ctc]
 
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Ctc Ctc]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Ctc Ctc]
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|colspan="2" | '''Genetic context''' <br/> [[Image:ctc_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ctc_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ctc_58783_59397_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ctc_expression.png|500px]]
 
 
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** ''[[ctc]]'' [http://www.ncbi.nlm.nih.gov/pubmed/6790515 PubMed]
 
** ''[[ctc]]'' [http://www.ncbi.nlm.nih.gov/pubmed/6790515 PubMed]
  
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ctc_58783_59397_1 ctc] {{PubMed|22383849}}
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* '''[[Sigma factor]]:'''  
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* '''Sigma factor:'''  
 
 
** ''[[gcaD]]'': [[SigA]] [http://www.ncbi.nlm.nih.gov/pubmed/8522540 PubMed]
 
** ''[[gcaD]]'': [[SigA]] [http://www.ncbi.nlm.nih.gov/pubmed/8522540 PubMed]
 
** ''[[ctc]]'': [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/6790515 PubMed]
 
** ''[[ctc]]'': [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/6790515 PubMed]

Revision as of 10:13, 7 August 2012

Gene name ctc
Synonyms
Essential no
Product ribosomal protein
Function unknown
Interactions involving this protein in SubtInteract: Ctc
Metabolic function and regulation of this protein in SubtiPathways:
Nucleotides (regulation), Murein recycling
MW, pI 21 kDa, 4.216
Gene length, protein length 612 bp, 204 aa
Immediate neighbours prs, spoVC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Ctc context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

translation, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU00520

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: Ctc subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1FEU (from Thermus thermophilus, 32% identity, 53% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant: GP500(spc), available in Stülke lab
  • Expression vector:
    • expression/ purification with N-terminal His-tag in E. coli: pGP804 (in pWH844), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody: available in Stülke lab

Labs working on this gene/protein

Your additional remarks

References

Reviews

Additional reviews: PubMed


Original publications

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Matthias Schmalisch, Ines Langbein, Jörg Stülke
The general stress protein Ctc of Bacillus subtilis is a ribosomal protein.
J Mol Microbiol Biotechnol: 2002, 4(5);495-501
[PubMed:12432960] [WorldCat.org] (P p)

R Fedorov, V Meshcheryakov, G Gongadze, N Fomenkova, N Nevskaya, M Selmer, M Laurberg, O Kristensen, S Al-Karadaghi, A Liljas, M Garber, S Nikonov
Structure of ribosomal protein TL5 complexed with RNA provides new insights into the CTC family of stress proteins.
Acta Crystallogr D Biol Crystallogr: 2001, 57(Pt 7);968-76
[PubMed:11418764] [WorldCat.org] [DOI] (P p)

I Hilden, B N Krath, B Hove-Jensen
Tricistronic operon expression of the genes gcaD (tms), which encodes N-acetylglucosamine 1-phosphate uridyltransferase, prs, which encodes phosphoribosyl diphosphate synthetase, and ctc in vegetative cells of Bacillus subtilis.
J Bacteriol: 1995, 177(24);7280-4
[PubMed:8522540] [WorldCat.org] [DOI] (P p)

C L Truitt, E A Weaver, W G Haldenwang
Effects on growth and sporulation of inactivation of a Bacillus subtilis gene (ctc) transcribed in vitro by minor vegetative cell RNA polymerases (E-sigma 37, E-sigma 32).
Mol Gen Genet: 1988, 212(1);166-71
[PubMed:2836704] [WorldCat.org] [DOI] (P p)

C Ray, M Igo, W Shafer, R Losick, C P Moran
Suppression of ctc promoter mutations in Bacillus subtilis.
J Bacteriol: 1988, 170(2);900-7
[PubMed:3123466] [WorldCat.org] [DOI] (P p)

M M Igo, R Losick
Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis.
J Mol Biol: 1986, 191(4);615-24
[PubMed:3100810] [WorldCat.org] [DOI] (P p)

J F Ollington, W G Haldenwang, T V Huynh, R Losick
Developmentally regulated transcription in a cloned segment of the Bacillus subtilis chromosome.
J Bacteriol: 1981, 147(2);432-42
[PubMed:6790515] [WorldCat.org] [DOI] (P p)

W G Haldenwang, R Losick
A modified RNA polymerase transcribes a cloned gene under sporulation control in Bacillus subtilis.
Nature: 1979, 282(5736);256-60
[PubMed:116131] [WorldCat.org] [DOI] (P p)

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